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Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

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Overview

Product name

Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end
See all Angiotensin Converting Enzyme 1 products (17) ...

Description

Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Carboxyterminal end

Specificity

This antibody is specific for Angiotensin Converting Enzyme 1.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide based on the carboxy end of the second peptidase unit of human Angiotensin Converting Enzyme 1.

Read Abcam's proprietary immunogen policy

(Peptide available as ab41280.)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This antibody has been peptide-affinity purified.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab39172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects a band of approximately 175-184kDa, due to glycosylation and other post-translational modifications (predicted molecular weight: 150 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Cellular localization

Cell Membrane

Alternative names

  • ACE 1 antibody
  • ACE 1 antibody
  • ACE antibody
  • ACE antibody
  • ACE T antibody
  • ACE T antibody
  • ACE1 antibody
  • ACE1 antibody
  • Angiotensin converting enzyme somatic isoform antibody
  • Angiotensin converting enzyme somatic isoform antibody
  • Angiotensin converting enzyme testis specific isoform antibody
  • Angiotensin converting enzyme testis specific isoform antibody
  • Angiotensin I converting enzyme 1 antibody
  • Angiotensin I converting enzyme antibody
  • Angiotensin I converting enzyme antibody
  • Angiotensin I converting enzyme peptidyl dipeptidase A 1 antibody
  • Carboxycathepsin antibody
  • Carboxycathepsin antibody
  • CD 143 antibody
  • CD143 antibody
  • CD143 antibody
  • CD143 antigen antibody
  • DCP 1 antibody
  • DCP 1 antibody
  • DCP antibody
  • DCP1 antibody
  • DCP1 antibody
  • Dipeptidyl carboxypeptidase 1 antibody
  • Dipeptidyl carboxypeptidase 1 antibody
  • Kininase II antibody
  • Kininase II antibody
  • MGC26566 antibody
  • MVCD3 antibody
  • Peptidase P antibody
  • Peptidase P antibody
  • Peptidyl dipeptidase A antibody
  • Peptidyl dipeptidase A antibody
  • Testicular ECA antibody
see all

Database links

see all

Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end images:

  Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

All lanes : Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172) at 1/1000 dilution

Lane 1 : ACE1 at 0.05 µg
Lane 2 : ACE1 at 0.01 µg
Lane 3 : ACE1 at 0.001 µg


Predicted band size : 150 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)

Protocols

  • Recommended WB protocol

    • References for Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end (ab39172)

    This product has been referenced in:

    • Boettger Tet al. Acquisition of the contractile phenotype by murine arterial smooth muscle cells depends on the Mir143/145 gene cluster. J Clin Invest 119:2634-47 (2009). WB; Mouse.Read more (PubMed: 19690389) »

    See 1 publication for this product

    Publishing research using ab39172? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"