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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end catalytic domain
See all Angiotensin Converting Enzyme 1 products (17) ...
Description
Rabbit polyclonal to Angiotensin Converting Enzyme 1 - Carboxyterminal end catalytic domain
Specificity
ab28313 recognizes metallopeptidase ACE1.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the amino end of the second peptidase unit of human ACE1.
(Peptide available as ab41279.)
Positive control
ACE1 is found in the lung, kidney, heart, pancreas, prostate, testis, small intestine and colon, and is found in cell lysates and as shed or processed forms in cell culture media and in biological fluids.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxy catalytic domain (ab28313)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 150 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Cellular localization
Cell Membrane
Alternative names
- ACE 1 antibody
- ACE 1 antibody
- ACE antibody
see all
Database links
- Entrez Gene: 1636 Human
- Omim: 106180 Human
- SwissProt: P12821 Human
- Unigene: 298469 Human
Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end catalytic domain images:
Western blot - Angiotensin Converting Enzyme 1 antibody - Carboxy catalytic domain (ab28313)
Lane 1 : Molecular weight markers
Lanes 2 - 4 : Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end catalytic domain (ab28313) at 1/1000 dilution
Lane 1 : As above
Lane 2 : ACE1 at 0.05 µg
Lane 3 : ACE1 at 0.01 µg
Lane 4 : ACE1 at 0.001 µg
Predicted band size : 150 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Angiotensin Converting Enzyme 1 antibody - Carboxyterminal end catalytic domain (ab28313)
ab28313 has not yet been referenced specifically in any publications.
Publishing research using ab28313? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"