![Western blot - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](#ab75762wb.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](#ab75762ihc1.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](#ab75762ihc2.gif)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757]
See all Angiotensin Converting Enzyme 1 products (17) ...
Description
Rabbit monoclonal [EPR2757] to Angiotensin Converting Enzyme 1
Tested applications
WB, IHC-P, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Does not react with
Rat
Immunogen
A synthetic peptide corresponding to residues on the C terminus of human Angiotensin Converting Enzyme 1
Positive control
Fetal kidney, fetal heart and fetal lung lysates; human kidney and spleen tissues.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 50mM Tris glycine, 0.15mM Sodium chloride, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2757
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Signal Transduction >> Metabolism >> Vitamins / Minerals
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Blood >> Serum Proteins
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Western blot - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
![Western blot - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](/ps/datasheet/Images/75/ab75762/ab75762wb.gif)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](/ps/datasheet/Images/75/ab75762/ab75762ihc1.gif)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) image](/ps/datasheet/Images/75/ab75762/ab75762ihc2.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 195 kDa (predicted molecular weight: 150 kDa).
IHC-P: 1/100 - 1/250.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Flow Cyt: 1/30Flow Cyt: 1/30
Application notes
Is unsuitable for ,ICC or IP.
Target
Relevance
ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.
Cellular localization
Cell Membrane
Alternative names
- ACE 1 antibody
- ACE antibody
- ACE T antibody
see all
Database links
- Entrez Gene: 1636 Human
- Entrez Gene: 11421 Mouse
- Omim: 106180 Human
- SwissProt: P12821 Human
- SwissProt: P09470 Mouse
- Unigene: 298469 Human
Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] images:
Western blot - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
All lanes : Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762) at 1/1000 dilution
Lane 1 : fetal kidney lysate
Lane 2 : fetal heart lysate
Lane 3 : fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 150 kDa
Observed band size : 195 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ab75762 at 1/100 dilution staining Angiotensin Converting Enzyme 1 in human spleen by Immunohistochemistry, Paraffin-embedded tissue. Note positive staining of endothelial cells.
References for Anti-Angiotensin Converting Enzyme 1 antibody [EPR2757] (ab75762)
ab75762 has not yet been referenced specifically in any publications.
Publishing research using ab75762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"