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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Overview
Product name
Anti-Angiotensin Converting Enzyme 2 antibody - Catalytic domain
See all Angiotensin Converting Enzyme 2 products (14) ...
Description
Rabbit polyclonal to Angiotensin Converting Enzyme 2 - Catalytic domain
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the catalytic domain of Human Angiotensin Converting Enzyme 2.
(Peptide available as ab41282.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
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Western blot - Angiotensin Converting Enzyme 2 antibody - Catalytic domain (ab38888)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 97 kDa. Glycosylation and other post-translational modifications make the apparent molecular weight on SDS PAGE gels larger, about 120 kDa by Western blot. Higher concentrations of antibody may be needed for samples from more distantly related species. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
Sequence similarities
Belongs to the peptidase M2 family.
Post-translational
modifications
N-glycosylation on Asn-90 may limit SARS infectivity.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9BYF1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Database links
- Entrez Gene: 59272 Human
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- Unigene: 178098 Human
Anti-Angiotensin Converting Enzyme 2 antibody - Catalytic domain images:
Western blot - Angiotensin Converting Enzyme 2 antibody - Catalytic domain (ab38888)
All lanes : Anti-Angiotensin Converting Enzyme 2 antibody - Catalytic domain (ab38888) at 1/1000 dilution
Lane 1 : ACE2 at 0.05 µg
Lane 2 : ACE2 at 0.01 µg
Lane 3 : ACE2 at 0.001 µg
Predicted band size : 97 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa (possible cleavage fragment).
Protocols
References for Anti-Angiotensin Converting Enzyme 2 antibody - Catalytic domain (ab38888)
ab38888 has not yet been referenced specifically in any publications.
Publishing research using ab38888? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"