Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
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I'm wondering if Abcam offers antibody conjugation services. I'm interested in having an Alexa Fluor secondary conjugated to your anti-ACE2 primary antibody (ab15348). I realize that you sell kits to do this, however, I was wondering if this can be done by your company for us? |
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ANSWER: |
Thank you for contacting us. We do not offer conjugation services, unfortunately, but rather offer the conjugation kits (www.abcam.com/EasyLink). However, we do not carry Alexa Fluor conjugation kits or Alexa Fluor conjugated secondary antibodies. We do carry the comparable DyLight conjugated secondary antibodies which have the same photostability as the Alexa Fluors. We do carry Dylight 488, 550, 594, and 650 for the anti-rabbit secondaries (e.g. ab96891). Please let me know which application you need the secondary or conjugation kit for, as well which wavelength you will need to detect, and I am happy to assist you further. I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC
ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25ºC. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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