Angiotensin Converting Enzyme 2 antibody (ab15348)

Product Name 

Angiotensin Converting Enzyme 2 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Angiotensin Converting Enzyme 2

Immunogen 

Synthetic peptide: CKGENNPGFQNTDDVQTSF, corresponding to C terminal amino acids 788-805 of Human ACE2.
(Peptide available as ab15352.)
BLAST 'CKGENNPGFQNTDDVQTSF' with or

Reacts with 
(species key)

Hu, Cat, Frt, Mcq

Tested applications 
(see key)

IHC-P, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Angiotensin Converting Enzyme 2 peptide (788-805) (ab15352).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human kidney or human testis cell lysate

Cellular localization 

Cell membrane; single pass type I membrane protein and secreted (processed form).

Research areas 

Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs

Relevance 

Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22.

No cross reactivity to ACE1.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS with 0.02% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

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References for Angiotensin Converting Enzyme 2 antibody (ab15348)

  This product has been used in: (two most recent references)

Wang G et al. Discrepancy between intrarenal messenger RNA and protein expression of ACE and ACE2 in human diabetic nephropathy. Am J Nephrol 29:524-31 (2009). WB, IHC-P; Human. PubMed: 19077419

van den Brand JM et al. Pathology of experimental SARS coronavirus infection in cats and ferrets. Vet Pathol 45:551-62 (2008). PubMed: 18587105

See all 3 publication references for this product.


If you publish research using ab15348 please let us know so that we can cite the reference on this datasheet.

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