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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Overview
Product name
Anti-Angiotensin Converting Enzyme 2 antibody
See all Angiotensin Converting Enzyme 2 products (14) ...
Description
Rabbit polyclonal to Angiotensin Converting Enzyme 2
Tested applications
IHC-Fr, ICC/IF, WB, IHC-P, IHC-Pmore details
Cross reactivity
Reacts with
Cat, Human, Ferret, Macaque Monkey
Immunogen
Synthetic peptide: CKGENNPGFQNTDDVQTSF, corresponding to C terminal amino acids 788-805 of Human ACE2. (Peptide available as ab15352.)
CKGENNPGFQ NTDDVQTSF
Positive control
Human kidney or human testis cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
No cross reactivity to ACE1.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
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Western blot - Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 2 antibody (ab15348)
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Immunocytochemistry/ Immunofluorescence-Angiotensin Converting Enzyme 2 antibody(ab15348)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa).Can be blocked with Angiotensin Converting Enzyme 2 peptide (788-805) (ab15352).
IHC-P: Use at an assay dependent concentration.
IHC-P: Use a concentration of 2 µg/ml
Target
Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
Sequence similarities
Belongs to the peptidase M2 family.
Post-translational
modifications
N-glycosylation on Asn-90 may limit SARS infectivity.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9BYF1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Database links
- Entrez Gene: 59272 Human
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- Unigene: 178098 Human
Anti-Angiotensin Converting Enzyme 2 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Angiotensin Converting Enzyme 2 antibody (ab15348)
ab15348 at 2µg/ml staining Angiotensin Converting Enzyme 2 in human kidney by IHC
Immunocytochemistry/ Immunofluorescence-Angiotensin Converting Enzyme 2 antibody(ab15348)
ICC/IF image of ab15348 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15348, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Frozen sections) - Angiotensin Converting Enzyme 2 antibody (ab15348)
ab15348 staining angiotensin converting enzyme 2 in human thoracic aortic sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized and blocked with a proprietary kit. Samples were incubated with primary antibody (2 µg/ml in TBST + Sodium azide) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Angiotensin Converting Enzyme 2 antibody (ab15348)
This product has been referenced in:
- Smits SLet al. Distinct severe acute respiratory syndrome coronavirus-induced acute lung injury pathways in two different nonhuman primate species. J Virol 85:4234-45 (2011).Read more (PubMed: 21325418) »
- Wang Get al. Discrepancy between intrarenal messenger RNA and protein expression of ACE and ACE2 in human diabetic nephropathy. Am J Nephrol 29:524-31 (2009). WB, IHC-P; Human.Read more (PubMed: 19077419) »
See all 4 publications for this product
Publishing research using ab15348? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"