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Western blot - Angiotensin Converting Enzyme 2 antibody (ab87436)

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Product Name
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Angiotensin Converting Enzyme 2 antibody
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See all Angiotensin Converting Enzyme 2 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Angiotensin Converting Enzyme 2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Angiotensin Converting Enzyme 2.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cat, Dog, Hm, Orngt (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following tissue lysate: Human Kidney
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Cellular localization
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Cell membrane; single pass type I membrane protein and secreted (processed form).
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Research areas
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Cardiovascular >> Vasculature >> Vasoconstriction >> Other Cardiovascular >> Heart >> Contractility >> Inotropics Cardiovascular >> Blood >> Blood Pressure regulation Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
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Relevance
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Angiotensin converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1 7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. ACE1 inhibitors such as catopril and lisinopril do not block ACE2 activity. The full length sequence for ACE2 encodes an 805 amino acid protein with predicted mass of 92.46 kDa and a pI of 5.22.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Angiotensin Converting Enzyme 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Angiotensin Converting Enzyme 2 antibody images, references, products related to ab87436 and other tools.
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Angiotensin Converting Enzyme 2 antibody images:
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Western blot - Angiotensin Converting Enzyme 2 antibody (ab87436)
Angiotensin Converting Enzyme 2 antibody (ab87436) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 92 kDa Observed band size : 92 kDa Additional bands at : 115 kDa (possible glycosylated form).
Exposure time : 30 seconds
ACE 2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
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Search PubMed (MEDLINE) for references to Angiotensin Converting Enzyme 2
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Angiotensin Converting Enzyme 2 antibody - more information
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