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Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
Overview
Product name
Anti-Angiotensin Converting Enzyme 2 antibody
See all Angiotensin Converting Enzyme 2 products (14) ...
Description
Rabbit polyclonal to Angiotensin Converting Enzyme 2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Hamster, Cat, Dog, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Angiotensin Converting Enzyme 2.
(Peptide available as ab87437.)
Positive control
This antibody gave a positive signal in the following tissue lysate: Human Kidney
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Vasculature >> Vasoconstriction >> Other
Cardiovascular >> Heart >> Contractility >> Inotropics
Cardiovascular >> Blood >> Blood Pressure regulation
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> ACEs
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Western blot - Angiotensin Converting Enzyme 2 antibody (ab87436)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IHC-P: Use at an assay dependent concentration.
Target
Function
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
Tissue specificity
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
Sequence similarities
Belongs to the peptidase M2 family.
Post-translational
modifications
N-glycosylation on Asn-90 may limit SARS infectivity.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ9BYF1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
see all
Database links
- Entrez Gene: 59272 Human
- Entrez Gene: 70008 Mouse
- Entrez Gene: 302668 Rat
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- SwissProt: Q8R0I0 Mouse
- SwissProt: Q5EGZ1 Rat
- Unigene: 178098 Human
see all
Anti-Angiotensin Converting Enzyme 2 antibody images:
Western blot - Angiotensin Converting Enzyme 2 antibody (ab87436)
Anti-Angiotensin Converting Enzyme 2 antibody (ab87436) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 92 kDa
Observed band size : 92 kDa
Additional bands at : 115 kDa (possible glycosylated form).
Exposure time : 30 seconds
ACE 2 contains multiple glycosylation sites (SwissProt) which may account for the characteristic reaction observed around the predicted molecular weight.
References for Anti-Angiotensin Converting Enzyme 2 antibody (ab87436)
ab87436 has not yet been referenced specifically in any publications.
Publishing research using ab87436? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"