Angiotensin II Human ELISA kit (ab108796)

Overview

  • Product nameAngiotensin II Human ELISA kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.06 ng/ml
  • Range
    0.063 ng/ml - 1 ng/ml
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Angiotensin II Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of angiotensin II in plasma, serum, and cell culture supernatants.

    An angiotensin II specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an angiotensin II specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of angiotensin II captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • AGT
    • Ang II
    • Angiotensin 2
    • Angiotensinogen
    • ANHU
    • Serpin peptidase inhibitor clade A member 8
    • SERPINA8
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Angiotensin II Human ELISA kit images

  • hu Angiotensin II measured in biological fluids showing quantity (pg) per mL of tested sample

  • Standard curve : mean of duplicates (+/-SD) with background readings subtracted

Protocols

References for Angiotensin II Human ELISA kit (ab108796)

ab108796 has not yet been referenced specifically in any publications.

Product Wall

We have tested pig samples with this kit and noticed significant cross-reactivity. So pig samples should work with this kit.

ab108796 did not work for rat samples when tested in the laboratory and thus is not recommended for use with rat samples.

Abreview for Angiotensin II Human ELISA kit

Average Average 3/5 (Ease of Use)
Abreviews

Sample: Human Serum (Blood serum)
Specification: Blood serum
Amount of Sample: 10 µg
Type of Kit Used: ELISA
Positive control: QC control
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

Submitted Apr 29 2013

Thank you for your enquiry.


The lab replied with the following information:


Usually we use a 4-parameter trendline, but if that option isn’t available then a polynomial one will do fine. The factor 4 or 5 polynomia...

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Thank you for your patience in this matter.

I have talked to the lab and they have told me that you need to adjust pH to be able to dissolve EDTA. Probably PH 8.3-8.5 will work well. Check EDTA manufacturer datasheet to make sure.

P...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"