Angiotensin II Human ELISA kit (ab108796)
- Product nameAngiotensin II Human ELISA kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.9%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity= 0.06 ng/ml
- Range0.063 ng/ml - 1 ng/ml
- Assay time5h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Angiotensin II Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of angiotensin II in plasma, serum, and cell culture supernatants.
An angiotensin II specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an angiotensin II specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of angiotensin II captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 80X Biotinylated Human Angiotensin II Antibody 1 x 100µl Angiotensin II Microplate (12 x 8 well strips) 1 unit Angiotensin II Standard 1 vial Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- Ang II
- Angiotensin 2
- Serpin peptidase inhibitor clade A member 8
- SwissProt: P01019 Human
Our Abpromise guarantee covers the use of ab108796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Angiotensin II Human ELISA kit (ab108796)
ab108796 has not yet been referenced specifically in any publications.