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Abcam’s Angiotensin II Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of angiotensin II in plasma, serum, and cell culture supernatants.
An angiotensin II specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an angiotensin II specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of angiotensin II captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|80X Biotinylated Human Angiotensin II Antibody||1 x 75µl|
|Angiotensin II Microplate (12 x 8 well strips)||1 unit|
|Angiotensin II Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
hu Angiotensin II measured in biological fluids showing quantity (pg) per mL of tested sample
Standard curve : mean of duplicates (+/-SD) with background readings subtracted
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"