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- Proteins and Peptides
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Product name
Anti-Angiotensin II Type 1 Receptor antibody
See all Angiotensin II Type 1 Receptor products (11) ...
Description
Rabbit polyclonal to Angiotensin II Type 1 Receptor
Specificity
This antibody recognizes isoforms AT-1A and AT-1B of the Angiotensin II type 1 receptor (AT-1r) in rat.
Tested applications
WB, IHC-Fr, IHC-FoFr, Electron Microscopymore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide: PSDNMSSSAKKPASC conjugated to KLH, corresponding to Internal sequence amino acids 341-355 of Angiotensin II Type 1 Receptor AT-1A. Synthetic peptide: SSSAKKSASFFEVE conjugated to KLH, corresponding to Internal sequence amino acids 346-359 of Angiotensin II Type 1 Receptor AT-1B.
PSDNMSSSAKKPASC
SSSAKKSASFFEVE
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Antisera was affinity purified by passage through two affinity columns, one cross-linked to AT-1A and the other with AT-1B, resulting in specificity to the sequence PSDNMSSSAKKPASCFEVE (341-359).
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cardiovascular >> Blood >> Blood Pressure regulation
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
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Western blot - Angiotensin II Type 1 Receptor antibody (ab18801)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-FoFr
Electron Microscopy
Application notes
EM: 1/500.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 19706421).
WB: Use at an assay dependent dilution. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Tissue specificity
Liver, lung, adrenal and adrenocortical adenomas.
Involvement in disease
Defects in AGTR1 are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Post-translational
modifications
C-terminal Ser or Thr residues may be phosphorylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP30556
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AG2S antibody
- Agtr 1 antibody
- Agtr1 antibody
see all
Database links
- Entrez Gene: 281607 Cow
- Entrez Gene: 11607 Mouse
- Entrez Gene: 11608 Mouse
- Entrez Gene: 24180 Rat
- Entrez Gene: 81638 Rat
- SwissProt: P25104 Cow
- SwissProt: P29754 Mouse
- SwissProt: P29755 Mouse
see all
Anti-Angiotensin II Type 1 Receptor antibody images:
Western blot - Angiotensin II Type 1 Receptor antibody (ab18801)
All lanes : Anti-Angiotensin II Type 1 Receptor antibody (ab18801) at 1/800 dilution
Lane 1 : Dog heart whole tissue lysate at 50 µg
Lane 2 : Dog heart whole tissue lysate at 25 µg
Lane 3 : Dog heart whole tissue lysate at 10 µg
Lane 4 : Dog heart whole tissue lysate at 5 µg
Lane 5 : Dog heart whole tissue lysate at 2 µg
Secondary
HRP conjugated donkey polycloanl antibody
Performed under reducing conditions.
Predicted band size : 41 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Additional bands at : 32 kDa (possible non-specific binding).
Exposure time : 2 minutes
This image is courtesy of an Abreview submitted by Dr sudhish mishra
Protocols
References for Anti-Angiotensin II Type 1 Receptor antibody (ab18801)
This product has been referenced in:
- Wang Y et al. Drug target prediction based on the herbs components: the study on the multitargets pharmacological mechanism of qishenkeli acting on the coronary heart disease. Evid Based Complement Alternat Med 2012:698531 (2012). WB, IHC-P.Read more (PubMed: 22474514) »
- Giani JF et al. Angiotensin-(1-7) improves cardiac remodeling and inhibits growth-promoting pathways in the heart of fructose-fed rats. Am J Physiol Heart Circ Physiol 298:H1003-13 (2010).Read more (PubMed: 20061544) »
See all 7 publications for this product
Publishing research using ab18801? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"