Anti-Angiotensin II Type 2 Receptor antibody (ab19134)

Thank you for your reply. Before I give all the details that you requested I just wanted to say that abcam is my first choice in terms of antibodies and I have not been disappointed so far.

1. The purchase order is: ######

2. Tissue type: Rat kidney cortex

3. Conditions: I have tried a range – 1to1000, 1to2000, 1to4000, 1to8000 dilutions, and I obtained linearity with 1to2000 dilution and 40ug, 60ug and 80ug of protein. I am running western with 60ug of protein and 1to2000 dilution of primary antibody. The incubation with primary antibody was indeed carried out overnight in 4oC.

4. Storage: On the delivery day I spin down (in 4oC) and aliquoted antibody to 5ul. Eppendorf tubes were additionally wrapt with paraffin film to prevent any losses. Tubes were kept at -20oC.

I would be grateful for another tube of antibody as I have still got more samples to run. If you need any additional information please let me know.

With best regards,

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement:

Order number: ######

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Dear Madam/Sir,

I have been using your ab19134 anti- AT2R (rabbit polyclonal primary antibody) for quite a long time. It worked beautifully until last week.

I ordered from you in the end of last year 2 tubes (2x50ug) of ab19134, both lot: GR-55294-1. The antibody was aliquoted to the required amount and stored as advised. Until recently I have been using aliquots from one tube and it was fine. This week I used antibody aliquots from the second tube and my western did not work properly. Western blot: I am using Amersham fluorescent double staining. On the same membrane, at the same time I scan for bands of AT2R and Histone. As you can see on the membrane scanned on 06-03-12 histone is fine (Please see attached images). The membrane from 01-03-12 is the good one but on the 06-03-12 I can barely see the band for AT2R. I did not change anything but the primary antibody. Since I treated both of the tubes in the same way from the arrival I suspect that the problem occurred before ab19134 was shipped. Therefore, would it be possible to get a refund or another 50ug of ab19134?

Regards,

ANSWER:

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital information for our monitoring of product quality. I would like to reassure you that this antibody is tested and covered by our guarantee forWB and Mouse, Rat, Cow andDog samples.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, as the othervial has worked well, I am sorry it will be difficult to provide further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.

If you have purchased within the guarantee period of 6 months and used the antibody on a tested and guaranteed species, I am pleased to offer you a free of charge replacement or credit note in compensation. In order to proceed with this, and also to provide some further information for quality monitoring purposes, I would appreciate if you could confirm the following:

1. The order number and date of purchase.

2. Which species was the sample from and from which tissue type or cell line?

3. Please confirm which concentration has the antibody been tried at. Has the incubation been tried overnight at 4oC?

4. What were the storage conditions for the antibody?

Thank you for your time and cooperation. I look forward to hearing from you with the requested information and details of how you would like to proceed.

 Dear Madam/Sir, I ordered ab19134 from you at November 2010 (last year), the order number is : ########. Could you find out for me what concentration had this batch of ab19134.   Additionally I would like to know what concentration have ab18801, which I ordered at August 2011 (this year), the order number is : ########.   Thank you for your help,      

ANSWER:

Thank you for your enquiry.

I can confirm the following concentrations:

ab18801 Angiotensin II Type 1 Receptor   2.1 mg/ml

ab19134 Angiotensin II Type 2 Receptor 1.6 mg/ml as stated on the datasheet

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Dear Madam/Sir,   I ordered recently Ang II Type 2 receptor peptide (ab91522). I couldn’t find any protocol that could guide me while using this product. Could you please advise on the conditions of the reaction and concentration of the peptide I should be using? With best Regards,

ANSWER:

Thank you for your enquiry and your interest in our products.

This peptide was used as an immunogen for ab19134 - Angiotensin II Type 2 Receptor antibody and it can be used in blocking experiments.

If you need any further assistance in the future, please do not hesitate to contact me.

I buy this antibody (ab19134) and there is no infomation about molecular weight. Can you send me information about this protein molecular weight.

ANSWER:

Thank you for your enquiry. The molecular weight of the Angiotensin II Type 2 Receptor target protein for this antibody is 41184.

I hope this is helpful. Please do not hesitate to contact me again should you have any further questions.