Overview

  • Product name
    Anisomycin
  • Description
    Protein synthesis inhibitor
  • Biological description
    Translational inhibitor of protein synthesis. Highly potent activator of kinase cascades. Potently activates stress-activated protein kinases, p38, JNK MAP and other kinases.
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Croons V  et al. The protein synthesis inhibitor anisomycin induces macrophage apoptosis in rabbit atherosclerotic plaques through p38 mitogen-activated protein kinase. J Pharmacol Exp Ther 329:856-64 (2009). Read more (PubMed: 19286921) »
  • Xiong W  et al. Anisomycin activates p38 MAP kinase to induce LTD in mouse primary visual cortex. Brain Res 1085:68-76 (2006). Read more (PubMed: 16581040) »
  • Cano E  et al. Anisomycin-activated protein kinases p45 and p55 but not mitogen-activated protein kinases ERK-1 and -2 are implicated in the induction of c-fos and c-jun. Mol Cell Biol 14:7352-62 (1994). Read more (PubMed: 7935449) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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