Overview

  • Product nameAnti-Ankyrin erythroid antibody
  • Description
    Rabbit polyclonal to Ankyrin erythroid
  • SpecificityThe 14 AA immnunogen for this antibody is present in isoform Er5, 6, 11, 12, 15, Mu17, Mu18 and Mu20, P16157. Mu17, 18 and 20 are 17 kd, 15 kd and 8 kd respectively. The antibody preferentially detects Mu17 and we are not sure whether it can detect other isoforms.
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Ankyrin erythroid (C terminal). The immunogen is a 14aa peptide within this sequence: RRQIDLSSAD AAQEHEEVEL RGSGLQPDLI EGRKGAQIVK RASLKRGKQ, corresponding to C terminal amino acids of Human Ankyrin erythroid
    Sequence:

    RRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

  • Positive control
    • Jurkat cell lysate Squamous epithelial cells of human skin Alveolar cells of human lung

Properties

Applications

Our Abpromise guarantee covers the use of ab58698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionAttaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.
    Isoform Mu17 together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.
  • Tissue specificityIsoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.
  • Involvement in diseaseDefects in ANK1 are a cause of spherocytosis type 1 (SPH1) [MIM:182900]; also called hereditary spherocytosis type 1 (HS1). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. Inheritance can be autosomal dominant or recessive.
  • Sequence similaritiesContains 23 ANK repeats.
    Contains 1 death domain.
    Contains 1 ZU5 domain.
  • DomainThe 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.
    The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.
  • Post-translational
    modifications
    Regulated by phosphorylation.
    Palmitoylated.
  • Cellular localizationCytoplasm > cytoskeleton. Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane; Sarcoplasmic reticulum and Membrane. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANK antibody
    • ANK-1 antibody
    • Ank1 antibody
    • ANK1_HUMAN antibody
    • Ankyrin 1 antibody
    • Ankyrin 1, erythrocytic antibody
    • Ankyrin R antibody
    • Ankyrin-1 antibody
    • Ankyrin-R antibody
    • Erythrocyte ankyrin antibody
    • SPH1 antibody
    • SPH2 antibody
    see all

Anti-Ankyrin erythroid antibody images

  • All lanes : Anti-Ankyrin erythroid antibody (ab58698) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 17 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 15%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Ankyrin erythroid with ab58698 at 4-8µg/ml. Arrows indicate positively labelled squamous epithelial cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Ankyrin erythroid with ab58698 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

References for Anti-Ankyrin erythroid antibody (ab58698)

This product has been referenced in:
  • Mortensen M  et al. The autophagy protein Atg7 is essential for hematopoietic stem cell maintenance. J Exp Med 208:455-67 (2011). IHC-P ; Mouse . Read more (PubMed: 21339326) »

See 1 Publication for this product

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