 image](#Annexin-A1-Primary-antibodies-ab4635-1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
Overview
Product name
Anti-Annexin A1 antibody [6E4/3]
See all Annexin A1 products (14) ...
Description
Mouse monoclonal [6E4/3] to Annexin A1
Tested applications
IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Mixture of native membrane proteins from human bone tissues. This includes native whole annexin proteins.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
6E4/3
Myeloma
Sp2
Isotype
IgG1
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Annexin A1 antibody [6E4/3](ab4635)
 image](/ps/datasheet/images/4/ab4635/Annexin-A1-Primary-antibodies-ab4635-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 38.5 kDa.
ELISA: Use at an assay dependent dilution.
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational
modifications
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
Target information above from: UniProt accessionP04083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Annexin A1 antibody
- Annexin I antibody
- Annexin-1 antibody
see all
Database links
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
Anti-Annexin A1 antibody [6E4/3] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Annexin A1 antibody [6E4/3](ab4635)
Ab4635 staining Human normal esophagus. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Annexin A1 antibody [6E4/3] (ab4635)
ab4635 has not yet been referenced specifically in any publications.
Publishing research using ab4635? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"