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Immunohistochemistry (Paraffin-embedded sections) - Annexin A1 antibody (ab33061)
Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.
Western blot - Annexin A1 antibody (ab33061)
Lane 1 : Anti-Annexin A1 antibody (ab33061) at 5 µg/ml
Lane 2 : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml
Lane 3 : Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml
Lane 1 : Cell lysate prepared from human HepG2 cells
Lane 2 : Cell lysate prepared from human HepG2 cells
Lane 3 : Cell lysate prepared from human HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 39 kDa
Western blot
Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 3 minutes
Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.
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