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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
Overview
Product name
Anti-Annexin A1 antibody
See all Annexin A1 products (14) ...
Description
Rabbit polyclonal to Annexin A1
Tested applications
ICC, WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cat, Chimpanzee
Immunogen
Synthetic peptide: MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA, corresponding to a region within N terminal amino acids 1-50 of Human Annexin A1.
MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA
Positive control
WB: HepG2 cell lysate. IHC-P: Human intestine.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependent dilution. (PubMed: 20618702Fixed with acetone.)
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 33 kDa (predicted molecular weight: 39 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
ELISA
ELISA: 1/62500ELISA: 1/62500
Target
Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational
modifications
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
Target information above from: UniProt accessionP04083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Annexin A1 antibody
- Annexin I antibody
- Annexin-1 antibody
see all
Database links
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
Anti-Annexin A1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Annexin A1 antibody (ab33061)
Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.
Western blot - Annexin A1 antibody (ab33061)
Lane 1 : Anti-Annexin A1 antibody (ab33061) at 5 µg/ml
Lane 2 : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml
Lane 3 : Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml
Lane 1 : Cell lysate prepared from human HepG2 cells
Lane 2 : Cell lysate prepared from human HepG2 cells
Lane 3 : Cell lysate prepared from human HepG2 cells
Lysates/proteins at 25 µg per lane.
Predicted band size : 39 kDa
Western blot
Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml + Annexin A1 protein (Human) (ab88803) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 3 minutes
Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.
References for Anti-Annexin A1 antibody (ab33061)
This product has been referenced in:
- Tyburczy MEet al. Novel Proteins Regulated by mTOR in Subependymal Giant Cell Astrocytomas of Patients with Tuberous Sclerosis Complex and New Therapeutic Implications. Am J Pathol 176:1878-90 (2010). IHC-P; Human.Read more (PubMed: 20133820) »
- Lilly EAet al. Annexin-A1 identified as the oral epithelial cell anti-Candida effector moiety. Mol Oral Microbiol 25:293-304 (2010). ICC; Human.Read more (PubMed: 20618702) »
See all 5 publications for this product
Publishing research using ab33061? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"