Overview

  • Product nameAnti-Annexin A1 antibody
    See all Annexin A1 primary antibodies
  • Description
    Rabbit polyclonal to Annexin A1
  • Tested applicationsICC, WB, IHC-P, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cat, Chimpanzee
  • Immunogen

    Synthetic peptide: MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA, corresponding to a region within N terminal amino acids 1-50 of Human Annexin A1.

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab33061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution. PubMed: 20618702Fixed with acetone.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
Flow Cyt Use at an assay dependent concentration. PubMed: 22378018

Target

  • FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
  • Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin 1 antibody
    • Annexin A1 antibody
    • Annexin I (lipocortin I) antibody
    • Annexin I antibody
    • Annexin-1 antibody
    • AnnexinA1 antibody
    • AnnexinI antibody
    • ANX 1 antibody
    • ANX A1 antibody
    • ANX1 antibody
    • ANXA 1 antibody
    • ANXA1 antibody
    • ANXA1 protein antibody
    • ANXA1_HUMAN antibody
    • Calpactin 2 antibody
    • Calpactin II antibody
    • Calpactin-2 antibody
    • CalpactinII antibody
    • Chromobindin 9 antibody
    • Chromobindin-9 antibody
    • Chromobindin9 antibody
    • HGNC:533 antibody
    • Lipocortin 1 antibody
    • Lipocortin I antibody
    • Lipocortin1 antibody
    • LipocortinI antibody
    • LPC 1 antibody
    • LPC1 antibody
    • p35 antibody
    • Phospholipase A2 inhibitory protein antibody
    see all

Anti-Annexin A1 antibody images



  • Predicted band size : 39 kDa
    Western blot analysis of HepG2 cell lysate labeling Annexin A1 with ab33061 at 1.25µg/ml.
  • Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.Ab33061, at 4 µg/ml, staining Annexin A1 in paraffin embedded human intestine tissue sections by Immuonhistochemistry. The arrows indicate the positively labelled cells (epithelial cells of intestinal glands). Magnification: 400X.
  • All lanes : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa
  • Lane 1 : Anti-Annexin A1 antibody (ab33061) at 5 µg/ml
    Lane 2 : Anti-Annexin A1 antibody (ab33061) at 2.5 µg/ml
    Lane 3 : Anti-Annexin A1 antibody (ab33061) at 1.25 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 39 kDa

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Ab33061 recognizes the tagged Annexin A1 protein which has an expected molecular weight of 68 kDa.

References for Anti-Annexin A1 antibody (ab33061)

This product has been referenced in:
  • Nuss JE  et al. Multi-Faceted Proteomic Characterization of Host Protein Complement of Rift Valley Fever Virus Virions and Identification of Specific Heat Shock Proteins, Including HSP90, as Important Viral Host Factors. PLoS One 9:e93483 (2014). WB . Read more (PubMed: 24809507) »
  • Tsai WH  et al. Annexin A1 mediates the anti-inflammatory effects during the granulocytic differentiation process in all-trans retinoic acid-treated acute promyelocytic leukemic cells. J Cell Physiol 227:3661-9 (2012). Flow Cyt . Read more (PubMed: 22378018) »

See all 7 Publications for this product

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