Overview
- Product nameAnti-Annexin A1 antibodySee all Annexin A1 primary antibodies ...
- DescriptionRabbit polyclonal to Annexin A1
- Tested applicationsWB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Human
Predicted to work with: a wide range of other species - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Annexin A1.
(Peptide available as ab47660.)
- Positive controlab47661 gave a positive signal in the following Human Whole Cell Lysates: MOLT4 and THP1. It also gave a positive signal in A549 cell line in ICC/IF.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab47661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).Can be blocked with Annexin A1 peptide (ab47660). |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
| IHC-P | IHC-P: 1/50. |
Target
- FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
- Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. - DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. - Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
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Database links
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
Target information above from: UniProt accession
P04083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Annexin 1 antibodyAnnexin A1 antibodyAnnexin I (lipocortin I) antibody
- Annexin I antibodyAnnexin-1 antibodyAnnexinA1 antibodyAnnexinI antibodyANX 1 antibodyANX A1 antibodyANX1 antibodyANXA 1 antibodyANXA1 antibodyANXA1 protein antibodyANXA1_HUMAN antibodyCalpactin 2 antibodyCalpactin II antibodyCalpactin-2 antibodyCalpactinII antibodyChromobindin 9 antibodyChromobindin-9 antibodyChromobindin9 antibodyHGNC:533 antibodyLipocortin 1 antibodyLipocortin I antibodyLipocortin1 antibodyLipocortinI antibodyLPC 1 antibodyLPC1 antibodyp35 antibodyPhospholipase A2 inhibitory protein antibody
see all
Anti-Annexin A1 antibody images
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Western blot - Annexin A1 antibody (ab47661)All lanes : Anti-Annexin A1 antibody (ab47661) at 1 µg/ml
Lane 1 :THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate (ab7913)
Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 39 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1 antibody (ab47661)ICC/IF image of ab47661 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47661, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Annexin A1 antibody (ab47661)
ab47661 has not yet been referenced specifically in any publications.
