Overview
- Product nameAnti-Annexin A1 antibodySee all Annexin A1 primary antibodies ...
- DescriptionGoat polyclonal to Annexin A1
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee - Immunogen
Synthetic peptide: CQAILDETKGDYEK, corresponding to C terminal amino acids 324-337 of Human Annexin A1 according to NP_000691.1
- Positive controlA431 lysate Rat spleen
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab81528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 39 kDa. |
Target
- FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
- Sequence similaritiesBelongs to the annexin family.
Contains 4 annexin repeats. - DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
- Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. - Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
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Database links
- Entrez Gene: 472953 Chimpanzee
- Entrez Gene: 327662 Cow
- Entrez Gene: 100728817 Guinea pig
- Entrez Gene: 100033940 Horse
- Entrez Gene: 301 Human
- Entrez Gene: 16952 Mouse
- Entrez Gene: 396942 Pig
- Entrez Gene: 100328962 Rabbit
- Entrez Gene: 25380 Rat
- Omim: 151690 Human
- SwissProt: A5A6M2 Chimpanzee
- SwissProt: P46193 Cow
- SwissProt: P14087 Guinea pig
- SwissProt: Q8HZM6 Horse
- SwissProt: P04083 Human
- SwissProt: P10107 Mouse
- SwissProt: P19619 Pig
- SwissProt: P51662 Rabbit
- SwissProt: P07150 Rat
- Unigene: 494173 Human
- Unigene: 248360 Mouse
- Unigene: 1792 Rat
see all
Target information above from: UniProt accession
P04083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Annexin 1 antibodyAnnexin A1 antibodyAnnexin I (lipocortin I) antibody
- Annexin I antibodyAnnexin-1 antibodyAnnexinA1 antibodyAnnexinI antibodyANX 1 antibodyANX A1 antibodyANX1 antibodyANXA 1 antibodyANXA1 antibodyANXA1 protein antibodyANXA1_HUMAN antibodyCalpactin 2 antibodyCalpactin II antibodyCalpactin-2 antibodyCalpactinII antibodyChromobindin 9 antibodyChromobindin-9 antibodyChromobindin9 antibodyHGNC:533 antibodyLipocortin 1 antibodyLipocortin I antibodyLipocortin1 antibodyLipocortinI antibodyLPC 1 antibodyLPC1 antibodyp35 antibodyPhospholipase A2 inhibitory protein antibody
see all
Anti-Annexin A1 antibody images
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Western blot - Anti-Annexin A1 antibody (ab81528)Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg
Predicted band size : 39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-Annexin A1 antibody (ab81528)Anti-Annexin A1 antibody (ab81528) at 0.5 µg/ml (for 1 hour) + A431 lysate in RIPA buffer at 35 µg
Predicted band size : 39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-Annexin A1 antibody (ab81528)
ab81528 has not yet been referenced specifically in any publications.
