Anti-Annexin A1 antibody (ab88060)

Overview

• Product nameAnti-Annexin A1 antibodySee all Annexin A1 primary antibodies ...
• Description
  Mouse monoclonal to Annexin A1
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full-length recombinant Human protein (AAH01275) with proprietary tag

• Positive controlLysate from HepG2 cells or placenta tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Annexins

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Other

Applications

Target

• FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
• Sequence similaritiesBelongs to the annexin family.
  Contains 4 annexin repeats.
• DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
• Post-translational
  modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
• Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Target information above from: UniProt accession P04083 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 301 Human
  • Omim: 151690 Human
  • SwissProt: P04083 Human
  • Unigene: 494173 Human

• Alternative names
  Annexin 1 antibodyAnnexin A1 antibodyAnnexin I (lipocortin I) antibody

  Annexin I antibodyAnnexin-1 antibodyAnnexinA1 antibodyAnnexinI antibodyANX 1 antibodyANX A1 antibodyANX1 antibodyANXA 1 antibodyANXA1 antibodyANXA1 protein antibodyANXA1_HUMAN antibodyCalpactin 2 antibodyCalpactin II antibodyCalpactin-2 antibodyCalpactinII antibodyChromobindin 9 antibodyChromobindin-9 antibodyChromobindin9 antibodyHGNC:533 antibodyLipocortin 1 antibodyLipocortin I antibodyLipocortin1 antibodyLipocortinI antibodyLPC 1 antibodyLPC1 antibodyp35 antibodyPhospholipase A2 inhibitory protein antibody

  see all

Anti-Annexin A1 antibody images

• 

  Western blot - Annexin A1 antibody [2D11] (ab88060)
  Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + immunogen (recombinant full length tagged protein) at 0.2 µg
  
  Secondary
  Goat Anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 39 kDa
  
• 

  Western blot - Annexin A1 antibody [2D11] (ab88060)
  Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + Human placenta lysate at 25 µg
  
  Secondary
  Goat Anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 39 kDa
  
• 

  Western blot - Annexin A1 antibody [2D11] (ab88060)
  Anti-Annexin A1 antibody (ab88060) at 5 µg/ml + HepG2 cell lysate at 50 µg
  
  Secondary
  Goat Anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 39 kDa
  
• 

  Western blot - Annexin A1 antibody [2D11] (ab88060)
  All lanes : Anti-Annexin A1 antibody (ab88060) at 5 µg/ml
  
  Lane 1 : transfected 293T cell line
  Lane 2 : non transfected 293T cell line
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG at 1/5000 dilution
  
  Predicted band size : 39 kDa