Anti-Annexin A1 antibody (ab88865)

Overview

• Product nameAnti-Annexin A1 antibodySee all Annexin A1 primary antibodies ...
• Description
  Mouse polyclonal to Annexin A1
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length Human Annexin A1 protein (NP_000691.1).

• Positive controlHuman spleen tissue lysate, Annexin A1 transfected 293T cell lysate, Human endometrium tissue and HeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Annexins

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Other

Applications

Target

• FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
• Sequence similaritiesBelongs to the annexin family.
  Contains 4 annexin repeats.
• DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
• Post-translational
  modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
• Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Target information above from: UniProt accession P04083 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 301 Human
  • Omim: 151690 Human
  • SwissProt: P04083 Human
  • Unigene: 494173 Human

• Alternative names
  Annexin 1 antibodyAnnexin A1 antibodyAnnexin I (lipocortin I) antibody

  Annexin I antibodyAnnexin-1 antibodyAnnexinA1 antibodyAnnexinI antibodyANX 1 antibodyANX A1 antibodyANX1 antibodyANXA 1 antibodyANXA1 antibodyANXA1 protein antibodyANXA1_HUMAN antibodyCalpactin 2 antibodyCalpactin II antibodyCalpactin-2 antibodyCalpactinII antibodyChromobindin 9 antibodyChromobindin-9 antibodyChromobindin9 antibodyHGNC:533 antibodyLipocortin 1 antibodyLipocortin I antibodyLipocortin1 antibodyLipocortinI antibodyLPC 1 antibodyLPC1 antibodyp35 antibodyPhospholipase A2 inhibitory protein antibody

  see all

Anti-Annexin A1 antibody images

• 

  Western blot - Annexin A1 antibody (ab88865)
  Anti-Annexin A1 antibody (ab88865) at 1 µg/ml + human spleen tissue lysate at 50 µg
  
  Predicted band size : 39 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
• 

  Western blot - Annexin A1 antibody (ab88865)
  All lanes : Anti-Annexin A1 antibody (ab88865) at 1 µg/ml
  
  Lane 1 : Annexin A1 transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  
  Predicted band size : 39 kDa
  Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Annexin A1 antibody (ab88865)
  ab88865, at 3 µg/ml staining Annexin A1 in formalin-fixed, paraffin-embedded human endometrium by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Annexin A1 antibody (ab88865)
  ab88865, at 10 µg/ml staining Annexin A1 in HeLa cells by Immunofluorescence.