Overview

  • Product nameAnti-Annexin A2 antibodySee all Annexin A2 primary antibodies ...
  • Description
    Rabbit polyclonal to Annexin A2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Annexin II.

    (Peptide available as ab41801.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab40943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Annexin A2 peptide (ab41801) or Human Annexin A2 peptide (ab41802).

Target

  • FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • P36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Anti-Annexin A2 antibody images

  • All lanes : Anti-Annexin A2 antibody (ab40943) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Annexin A2 antibody (ab40943)

This product has been referenced in:

See all 2 Publications for this product

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