Anti-Annexin A2 antibody (ab40943)

Overview

• Product nameAnti-Annexin A2 antibodySee all Annexin A2 primary antibodies ...
• Description
  Rabbit polyclonal to Annexin A2
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Xenopus laevis
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Annexin II.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab41801.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), HEK293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Annexins

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Binding Proteins

Applications

Target

• FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
• Sequence similaritiesBelongs to the annexin family.
  Contains 4 annexin repeats.
• DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
• Post-translational
  modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
  ISGylated.
• Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Target information above from: UniProt accession P07355 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 396297 Chicken
  • Entrez Gene: 282689 Cow
  • Entrez Gene: 403435 Dog
  • Entrez Gene: 302 Human
  • Entrez Gene: 12306 Mouse
  • Entrez Gene: 56611 Rat
  • NCBI: 4757756 Human
  • Omim: 151740 Human

  • SwissProt: P17785 Chicken
  • SwissProt: P04272 Cow
  • SwissProt: Q6TEQ7 Dog
  • SwissProt: Q8TBV2 Human
  • SwissProt: Q567R4 Human
  • SwissProt: P07355 Human
  • SwissProt: P07356 Mouse
  • SwissProt: Q07936 Rat
  • Unigene: 511605 Human
  • Unigene: 238343 Mouse
  • Unigene: 90546 Rat

  see all

• Alternative names
  Annexin A2 antibodyAnnexin II antibodyAnnexin-2 antibody

  ANX 2 antibodyANX2 antibodyANX2L4 antibodyANXA2 antibodyANXA2_HUMAN antibodyarylsulfatase B antibodyCAL1H antibodyCalpactin I heavy chain antibodycalpactin I heavy polypeptide (p36) antibodyCalpactin-1 heavy chain antibodychromobindin 8 antibodyChromobindin-8 antibodyLIP2 antibodyLipocortin II antibodyLPC2 antibodyLPC2D antibodyP36 antibodyP36 protein antibodyPAP-IV antibodyplacental anticoagulant protein IV antibodyProtein I antibody

  see all

Anti-Annexin A2 antibody images

• 

  Western blot - Annexin II antibody (ab40943)
  All lanes : Anti-Annexin A2 antibody (ab40943) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 38 kDa
  Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.