Overview

  • Product nameAnti-Annexin A2 antibodySee all Annexin A2 primary antibodies ...
  • Description
    Mouse monoclonal to Annexin A2
  • Tested applicationsWB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-340 of Human Annexin II

Properties

Applications

Our Abpromise guarantee covers the use of ab54771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 38 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCalcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Post-translational
    modifications
    Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
    ISGylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Annexin A2 antibody
    • Annexin II antibody
    • Annexin II, heavy chain antibody
    • Annexin-2 antibody
    • ANX 2 antibody
    • ANX2 antibody
    • ANX2L4 antibody
    • ANXA2 antibody
    • ANXA2_HUMAN antibody
    • arylsulfatase B antibody
    • CAL1H antibody
    • Calpactin I heavy chain antibody
    • calpactin I heavy polypeptide (p36) antibody
    • Calpactin I heavy polypeptide antibody
    • Calpactin-1 heavy chain antibody
    • chromobindin 8 antibody
    • Chromobindin-8 antibody
    • Epididymis secretory protein Li 270 antibody
    • HEL S 270 antibody
    • LIP2 antibody
    • Lipocortin II antibody
    • LPC2 antibody
    • LPC2D antibody
    • P36 antibody
    • P36 protein antibody
    • PAP-IV antibody
    • placental anticoagulant protein IV antibody
    • Protein I antibody
    see all

Anti-Annexin A2 antibody images

  • Annexin II antibody (ab54771) used in immunofluorescence at 10ug/ml on HeLa cells.


  • Predicted band size : 38 kDa
    Annexin II antibody (ab54771) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Anti-Annexin A2 antibody (ab54771) at 1/1000 dilution + Whole cell lysate prepared from human Mesothelial cell line MeT-5A at 5 µg

    Secondary
    HRP conjugated rabbit polyclonal to mouse IgG at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 seconds

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • ab54771 (2µg/ml) staining Annexin II in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of glandular epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-Annexin A2 antibody (ab54771)

This product has been referenced in:
  • Li Q  et al. Plasmin plays an essential role in amplification of psoriasiform skin inflammation in mice. PLoS One 6:e16483 (2011). WB ; Human . Read more (PubMed: 21311769) »
  • Maxwell GL  et al. Proteomic analysis of stage I endometrial cancer tissue: Identification of proteins associated with oxidative processes and inflammation. Gynecol Oncol : (2011). WB ; Human . Read more (PubMed: 21458040) »

See all 3 Publications for this product

Product Wall

Thank you for your inquiry.

Unfortunately, we did not experimentally and specifically test if the monomer or tetramer form of Annexin A2 are recognized by these antibodies.

Heterotetramer containing 2 light chains of S100A10/p11 and...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (Mesothelial cell line MeT-5A (ATCC CRL-9444))
Loading amount 5 µg
Specification Mesothelial cell line MeT-5A (ATCC CRL-9444)
Gel Running Conditions Reduced Denaturing (GE Healthcare, Excelgel 12.5 homogenous)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"