Anti-Annexin A1 antibody [5E4/1] (ab2487)

Overview

• Product nameAnti-Annexin A1 antibody [5E4/1]See all Annexin A1 primary antibodies ...
• Description
  Mouse monoclonal [5E4/1] to Annexin A1
• Tested applicationsELISA, WB, ICC/IF, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Horse, Dog, Human
  
• Immunogen

  Mixture of native membrane proteins from human bone tissues. This includes native whole annexin proteins.

• Positive controlEndothelial cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number5E4/1
• MyelomaSp2
• IsotypeIgG1
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Annexins

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Other

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Other

Applications

Target

• FunctionCalcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
• Sequence similaritiesBelongs to the annexin family.
  Contains 4 annexin repeats.
• DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
• Post-translational
  modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
• Cellular localizationNucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Target information above from: UniProt accession P04083 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100033940 Horse
  • Entrez Gene: 301 Human
  • Omim: 151690 Human
  • SwissProt: Q8HZM6 Horse
  • SwissProt: P04083 Human
  • Unigene: 494173 Human

• Alternative names
  Annexin 1 antibodyAnnexin A1 antibodyAnnexin I (lipocortin I) antibody

  Annexin I antibodyAnnexin-1 antibodyAnnexinA1 antibodyAnnexinI antibodyANX 1 antibodyANX A1 antibodyANX1 antibodyANXA 1 antibodyANXA1 antibodyANXA1 protein antibodyANXA1_HUMAN antibodyCalpactin 2 antibodyCalpactin II antibodyCalpactin-2 antibodyCalpactinII antibodyChromobindin 9 antibodyChromobindin-9 antibodyChromobindin9 antibodyHGNC:533 antibodyLipocortin 1 antibodyLipocortin I antibodyLipocortin1 antibodyLipocortinI antibodyLPC 1 antibodyLPC1 antibodyp35 antibodyPhospholipase A2 inhibitory protein antibody

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Anti-Annexin A1 antibody [5E4/1] images

• 

  Immunohistochemistry (Frozen sections) - Anti-Annexin A1 antibody [5E4/1] (ab2487)Image from Dakin SG et al., PLoS One. 2012;7(2):e32333. Epub 2012 Feb 22. Fig 11.; doi:10.1371/journal.pone.0032333; February 22, 2012, PLoS ONE 7(2): e32333.
  ab2487, staining Annexin A1 (green) in and sub-acutely injured equine superficial digital flexor tendons by Immunohistochemistry (Frozen sections).
  
  After warming cryosections at room temperature for 30 minutes, sections were rehydrated in 1% PBS-Tween 20 (PBS-T) and blocked in PBS containing 5% normal goat serum for 1 hour at room temperature. Sections were incubated with the primary antibody (1/100 in PBS + 5% normal goat serum) for 2 hours. An AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/300).
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Annexin A1 antibody [5E4/1] (ab2487)Image from Cao Z et al., Mol Cell Proteomics. 2008 Sep;7(9):1651-67. Epub 2008 May 25. Fig 7.; doi: 10.1074/mcp.M700155-MCP200; September 1, 2008 Molecular & Cellular Proteomics, 7, 1651-1667.
  Immunofluorescence analysis of MDCK cells, staining Annexin A1 with ab2487.