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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
Overview
Product name
Anti-Annexin A1 antibody [5E4/1]
See all Annexin A1 products (14) ...
Description
Mouse monoclonal [5E4/1] to Annexin A1
Tested applications
ELISA, WB, IF, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Dog, Human
Immunogen
Mixture of native membrane proteins from human bone tissues. This includes native whole annexin proteins.
Positive control
Endothelial cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
5E4/1
Myeloma
Sp2
Isotype
IgG1
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 38.5 kDa.
IF: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration.
Target
Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational
modifications
Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity.
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages.
Target information above from: UniProt accessionP04083
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Annexin A1 antibody
- Annexin I antibody
- Annexin-1 antibody
see all
Database links
- Entrez Gene: 476322 Dog
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
References for Anti-Annexin A1 antibody [5E4/1] (ab2487)
This product has been referenced in:
- Cao Zet al. Use of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis. Mol Cell Proteomics 7:1651-67 (2008). WB, ICC/IF; Dog.Read more (PubMed: 18504258) »
See 1 publication for this product
Publishing research using ab2487? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"