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Annexin II 293T Transfected Lysate - (positive control) (ab94080)

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Overview

  • Product nameAnnexin II 293T Transfected Lysate - (positive control)
  • General notesab94080 is a 293T cell transfected lysate in which Human Annexin II has been transiently over-expressed using a pCMV-Annexin II plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundDomain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. PTM: Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

    • Applications

    Our Abpromise guarantee covers the use of ab94080 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Annexin II 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Annexin II 293T Transfected Lysate (ab94080)
      SDS-PAGE - Annexin II 293T Transfected Lysate (ab94080)
      ab94080 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Annexin II 293T Transfected Lysate (ab94080)
      Western blot - Annexin II 293T Transfected Lysate (ab94080)
      All lanes : Anti-Annexin A2 antibody (ab54771) at 1/500 dilution

      Lane 1 : Annexin II 293T Transfected Lysate - Positive Control (ab94080)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

    References for Annexin II 293T Transfected Lysate - (positive control) (ab94080)

    ab94080 has not yet been referenced specifically in any publications.

    Publishing research using ab94080 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"