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Product name
Anti-Annexin A2 antibody
See all Annexin A2 products (9) ...
Description
Goat polyclonal to Annexin A2
Specificity
This antibody is expected to recognise both reported isoforms. NP_004030.1, NP_001002857.1and NP_001129487.1 are varients of isoform 2.
Tested applications
Cross reactivity
Reacts with
Human, Pig
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide: STVHEILCKLSLEGD, corresponding to N terminal amino acids 2-16 of Human Annexin II.
STVHEILCKLSLEGD
Positive control
HeLa and A549 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Annexins
-
Western blot - Annexin II antibody (ab2242)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).
Target
Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational
modifications
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.
Target information above from: UniProt accessionP07355
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Annexin A2 antibody
- Annexin II antibody
- Annexin-2 antibody
see all
Database links
- Entrez Gene: 282689 Cow
- Entrez Gene: 302 Human
- Entrez Gene: 12306 Mouse
- Entrez Gene: 406192 Pig
- Entrez Gene: 56611 Rat
- NCBI: 4757756 Human
- Omim: 151740 Human
- SwissProt: P04272 Cow
see all
Anti-Annexin A2 antibody images:
Western blot - Annexin II antibody (ab2242)
Anti-Annexin A2 antibody (ab2242) at 0.5 µg/ml + A431 lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 38 kDa
Primary incubated for 1 hour.
References for Anti-Annexin A2 antibody (ab2242)
This product has been referenced in:
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB; Human.Read more (PubMed: 18701577) »
- Glebov OO et al. Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells. Nat Cell Biol 8:46-54 (2006).Read more (PubMed: 16341206) »
See all 2 publications for this product
Publishing research using ab2242? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"