Annexin V 293T Transfected Lysate - Positive Control (ab76216)

Overview

  • Product nameAnnexin V 293T Transfected Lysate - Positive ControlSee all Annexin V lysates ...
  • General notesab76216 is a 293T cell transfected lysate in which Human Annexin V has been transiently over-expressed using a pCMV-Annexin V plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared, click here.
  • Tested applicationsWBmore details

Properties

  • BackgroundDisease: Defects in ANXA5 are associated with susceptibility to pregnancy loss, recurrent, type 3 (RPRGL3) [MIM:614391]. A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.

Applications

Our Abpromise guarantee covers the use of ab76216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent concentration.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Annexin V 293T Transfected Lysate - Positive Control images

    • ab76216 at 15μg/lane on an SDS-PAGE gel.

    • All lanes : Anti-Annexin V antibody (ab54775) at 1/500 dilution

      Lane 1 : Annexin V 293T Transfected Lysate - Positive Control (ab76216)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Annexin V 293T Transfected Lysate - Positive Control (ab76216)

    ab76216 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"