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Product name Annexin V 293T Transfected Lysate - Positive Control See all Annexin V lysates
General notes ab76216 is a 293T cell transfected lysate in which Human Annexin V has been transiently over-expressed using a pCMV-Annexin V plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed about how the transfected lysate was prepared, click here.
Tested applications WB more details Properties
Storage instructions Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Beta mercaptoethanol, 2% SDS, 10% Glycerol, 50mM Tris HCl, pH 6.8
Concentration information loading...
Purity Whole Cell Lysate
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Annexin V 293T Transfected Lysate - Positive Control images
SDS-PAGE - Annexin V Transfected Lysate (ab76216)
ab76216 at 15μg/lane on an SDS-PAGE gel.
Western blot - Annexin V Transfected Lysate (ab76216)
All lanes : Anti-Annexin V antibody ( ab54775) at 1/500 dilution Lane 1 : Annexin V 293T Transfected Lysate - Positive Control (ab76216) Lane 2 : 293T non-transfected lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
References for Annexin V 293T Transfected Lysate - Positive Control (ab76216)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"