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Products:Cell Biology >> Apoptosis >> Annexin V Reagents
Overview
Product name
Annexin V 293T Transfected Lysate - (positive control)
See all Annexin V products (2) ...
General notes
ab94073 is a 293T cell transfected lysate in which Human Annexin V has been transiently over-expressed using a pCMV-Annexin V plasmid. The lysate is provided in 1X Sample Buffer.Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applications
Properties
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage buffer
pH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration
Concentration information loading...
Purity
Whole Cell Lysate
Research areas
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Detection kits >> Annexin V
Cell Biology >> Apoptosis >> Annexin V Reagents
Background
Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid. Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats.
Applications
Show applications key
Our Abpromise guarantee covers the use of ab94073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Annexin V 293T Transfected Lysate - (positive control) images:
SDS-PAGE - Annexin V 293T Transfected Lysate - (positive control) (ab94073)
ab94073 at 15µg/lane on an SDS-PAGE gel.
Western blot - Annexin V 293T Transfected Lysate - (positive control) (ab94073)
All lanes : Anti-Annexin V antibody (ab54775) at 1/500 dilution
Lane 1 : Annexin V 293T Transfected Lysate - (positive control) (ab94073)
Lane 2 : 293T non transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
References for Annexin V 293T Transfected Lysate - (positive control) (ab94073)
ab94073 has not yet been referenced specifically in any publications.
Publishing research using ab94073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"