You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Annexin V-Biotin Apoptosis Detection Kit (ab14190)

Q&A (4)

Overview

  • Product nameAnnexin V-Biotin Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 30m
  • Product overview

    The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cyt more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Anchorin CIIAnnexin 5Annexin A5
        Annexin VAnnexin-5Annexin5AnnexinA5AnnexinVANX 5ANX A5ANX5ANXA5ANXA5_HUMANCalphobindin ICBP ICBP-IEndonexin IIENX 2ENX2Lipocortin VPAP IPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPP 4Pp4RPRGL3Thromboplastin inhibitorVAC alphaVAC-alphaVascular anticoagulant alphaVascular anticoagulant-alpha
      see all

    Applications

    Our Abpromise guarantee covers the use of ab14190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Flow Cyt Flow Cyt

    Protocols

    References for Annexin V-Biotin Apoptosis Detection Kit (ab14190)

    ab14190 has not yet been referenced specifically in any publications.

    Publishing research using ab14190 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab14190

    Displaying 1 - 4 of 4 results for Q&A

    10
    Votes: Highest First

    Inquiry: Hello, I am looking to see if I can get a quote for this item:Annexin V-Biotin Apoptosis Detection Kit (ab14190) and for the Streptavidin-FITC to label it with. I plan to use these reagents in an undergraduate laboratory course. Thank you for you...

    Read More

    Thank you very much for contacting us with your question.

    We unfortunately do not sell the streptavidin-FITC to use with this kit, however we do have streptavidin-HRP. Would this be suitable for you?

    The apoptosis kit ab14190 is $465pe...

    Read More

    If I end at the fixation step, my results are fine. If I permeabilize after that and do another antibody labeling then my annexin staining disappears. Does this have to do with the fluorophore or the permeabilization step, or another issue?

    Thank you for your enquiry. Annexin V staining is calcium dependent, therefore all buffers used after the annexin V staining step should contain calcium (1.8-2.5 mM). Fluorescence will fade gradually under the light, therefore any experiments perfo...

    Read More

    Regarding the Annexin V-Biotin Assay Protocol, it states for step 5 "Incubate at room temperature for 5 min in the dark." Can the incubation period be extended to 30 min at 37C? If yes, are there any additional protocol changes that would need to be chan...

    Read More

    The incubation time can be extended to 30 min. However, a longer incubation period can sometimes result in more dead cells, which can give false positive results. Generally you should limit it to 10 min. After incubation, you can put the cells on ice if y...

    Read More

    I am interested in using the Annexin V biotin kit for high throughput screening. I was wondering if I can detect Annexin V staining using a plate reader? Also, would it be possible to receive a pdf of the protocol because I was unable to access it onlin...

    Read More

    Thank you for your enquiry. All the information that we have regarding this product is located on the online datasheet. Here is the protocol, please let me know if you have any more questions.... Annexin V-Biotin Assay Protocol A. Incubation of cel...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"