Annexin V-Biotin Apoptosis Detection Kit (ab14190)

Overview

  • Product nameAnnexin V-Biotin Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 30m
  • Product overview

    The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cyt more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-Biotin Apoptosis Detection Kit (ab14190)

ab14190 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us with your question.

We unfortunately do not sell the streptavidin-FITC to use with this kit, however we do have streptavidin-HRP. Would this be suitable for you?

The apoptosis kit ab14190 is $46...

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Thank you for your enquiry. Annexin V staining is calcium dependent, therefore all buffers used after the annexin V staining step should contain calcium (1.8-2.5 mM). Fluorescence will fade gradually under the light, therefore any experiments pe...

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The incubation time can be extended to 30 min. However, a longer incubation period can sometimes result in more dead cells, which can give false positive results. Generally you should limit it to 10 min. After incubation, you can put the cells on ice i...

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Thank you for your enquiry. All the information that we have regarding this product is located on the online datasheet. Here is the protocol, please let me know if you have any more questions.... Annexin V-Biotin Assay Protocol A. Incubation of ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"