Overview

  • Product nameAnnexin V-Cy3 Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    Abcam's Annexin V-Cy3 Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cytmore details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-Cy3 Apoptosis Detection Kit (ab14142)

This product has been referenced in:
  • Xu P  et al. The different role of Notch1 and Notch2 in astrocytic gliomas. PLoS One 8:e53654 (2013). Flow Cyt . Read more (PubMed: 23349727) »
  • Zhou X  et al. Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status. Lab Invest 90:144-55 (2010). Read more (PubMed: 20048743) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. This kit can only be used with fresh cells and tissue samples. Any process that leads to membrane damage like fixation and/or freeze-thaw cycles makes the sample incompatible with this kit. So unfortunately it cannot be...

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Thank you for your enquiry. Annexin V assay can only be used with fresh tissue sample. Simply incubate annexin V with the tissue piece, then visualize it using confocal microscopy. Frozen or fixed tissue are not suitable for the assay. For frozen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"