Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent (ab14143)
Overview
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Product name
Annexin V/ANXA5-Cy3 Apoptosis Staining / Detection Reagent
See all Annexin V/ANXA5 reagents -
Tested applications
Suitable for: FM, Flow Cytmore details -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 1002 Annexin V-Cy3 Reagent.
Annexin V/ANXA5-Cy3 Apoptosis Detection Reagent ab14143 (previously known as Annexin V-Cy3 Reagent 1002) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-Cy3 reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy3 reagent is also available as part of Annexin V/ANXA5-Cy3 apoptosis kit ab14142 and Annexin V/ANXA5-Cy3 with SYTOX apoptosis kit ab14144.
For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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Related Products
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- 10X Annexin V/ANXA5 Binding Buffer (ab14084)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
- Annexin V/ANXA5-PE Apoptosis Staining / Detection Reagent (ab14154)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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Notes |
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FM
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab14143 has been referenced in 5 publications.
- Noble RJ et al. Paracrine Behaviors Arbitrate Parasite-Like Interactions Between Tumor Subclones. Front Ecol Evol 9:N/A (2021). PubMed: 35096847
- Schuhmann D et al. Interfering with interferon-? signalling in intestinal epithelial cells: selective inhibition of apoptosis-maintained secretion of anti-inflammatory interleukin-18 binding protein. Clin Exp Immunol 163:65-76 (2011). PubMed: 21078084
- Thuwanut P et al. The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from the flat-headed cat (Prionailurus planiceps). Theriogenology 76:115-25 (2011). PubMed: 21396691
- Simonin Y et al. Calpain activation by hepatitis C virus proteins inhibits the extrinsic apoptotic signaling pathway. Hepatology 50:1370-9 (2009). PubMed: 19711428
- Pu P et al. Downregulation of Wnt2 and beta-catenin by siRNA suppresses malignant glioma cell growth. Cancer Gene Ther : (2008). PubMed: 18949017