Overview

  • Product nameAnnexin V-Cy5 Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    Abcam's Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cyt more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-Cy5 Apoptosis Detection Kit (ab14150)

This product has been referenced in:
  • Courtois ET  et al. In Vitro and in Vivo Enhanced Generation of Human A9 Dopamine Neurons from Neural Stem Cells by Bcl-XL. J Biol Chem 285:9881-97 (2010). Read more (PubMed: 20106970) »
  • Glauser DL  et al. Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities. J Virol 84:3808-24 (2010). Read more (PubMed: 20106923) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. I can confirm the concentration of annexin V in the ab14150 kit is 30 ug/ml. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"