Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
Key features and details
- Assay type: Direct
- Platform: Flow cytometer, Fluorescence microscope
- Assay time: 10 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Annexin V-EGFP Apoptosis Staining / Detection Kit
See all Annexin V/ANXA5 kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Direct -
Assay time
0h 10m -
Product overview
Annexin V-EGFP Apoptosis Staining / Detection Kit ab14153 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy with a FITC filter.
EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K104 Annexin V-EGFP Apoptosis Kit. K104-100 is the same size as the 100 test size of ab14153.
Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.
For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests Annexin V-EGFP 1 x 500µl Binding Buffer 1 x 50ml Propidium iodide 1 x 500µl -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all
Associated products
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- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
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Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab14153 has been referenced in 7 publications.
- Song P et al. CNOT6: A Novel Regulator of DNA Mismatch Repair. Cells 11:N/A (2022). PubMed: 35159331
- Tatsumi T et al. Forced lipophagy reveals that lipid droplets are required for early embryonic development in mouse. Development 145:N/A (2018). PubMed: 29475974
- Lamprianou S et al. Glibenclamide Prevents Diabetes in NOD Mice. PLoS One 11:e0168839 (2016). PubMed: 28006000
- Jagannathan R et al. AJUBA LIM Proteins Limit Hippo Activity in Proliferating Cells by Sequestering the Hippo Core Kinase Complex in the Cytosol. Mol Cell Biol 36:2526-42 (2016). PubMed: 27457617
- Sayin VI et al. Zfp148 deficiency causes lung maturation defects and lethality in newborn mice that are rescued by deletion of p53 or antioxidant treatment. PLoS One 8:e55720 (2013). PubMed: 23405202
- Xiang Y et al. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nat Commun 4:1659 (2013). PubMed: 23552074
- Kaymaz BT et al. Methylprednisolone induces apoptosis by interacting with the JAK/STAT pathway in HL-60 and K-562 leukemic cells. Hematology 17:93-9 (2012). PubMed: 22664047