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Annexin V-EGFP Apoptosis Detection Kit (ab14153)

Q&A (1)

Overview

  • Product nameAnnexin V-EGFP Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cyt more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Anchorin CIIAnnexin 5Annexin A5
        Annexin VAnnexin-5Annexin5AnnexinA5AnnexinVANX 5ANX A5ANX5ANXA5ANXA5_HUMANCalphobindin ICBP ICBP-IEndonexin IIENX 2ENX2Lipocortin VPAP IPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPP 4Pp4RPRGL3Thromboplastin inhibitorVAC alphaVAC-alphaVascular anticoagulant alphaVascular anticoagulant-alpha
      see all

    Applications

    Our Abpromise guarantee covers the use of ab14153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Flow Cyt Flow Cyt

    Protocols

    References for Annexin V-EGFP Apoptosis Detection Kit (ab14153)

    ab14153 has not yet been referenced specifically in any publications.

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    Thanks you very much for your answer. I have an other question for you. In the AnnexinV-EGFP apoptosis detection kit (ab 14153), there are 500 µL Annexin V-eGFP and the kit provides approx. 100 assays. But in the protocol, 1 µL of AnnexinV-eGFP is u...

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    Thank you for your enquiry. I can tell you that there was a mistake in step 4 of the protocol; this should read "Add 5 µl of Annexin V-EGFP and 5 µl of propidium iodide" and not "1 µl of Annexin V-EGFP and 1 µl of propidium iodide". Therefore this kit ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"