Overview

  • Product nameAnnexin V-FITC Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    Abcam's Annexin V-FITC Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cytmore details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Annexin V-FITC Apoptosis Detection Kit images

  • Annexin V-FITC/ PI staining of AG06173 primary fibroblasts.
    105 cells were used for analysis. Resuspended cells were incubated with Annexin V-FITC for 15 min in the dark. Propidium iodide was used as a counterstain to discriminate necrotic/ dead cells from apoptotic cells. Left: negative control - AG6173 untreated cells. Right: positive control - AG6173 cells irradiated at 10 Gy.
    Image courtesy of S. Khoronenkova PhD, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, UK.

Protocols

References for Annexin V-FITC Apoptosis Detection Kit (ab14085)

This product has been referenced in:
  • Fan X  et al. Proteolysis of the human DNA polymerase delta smallest subunit p12 by µ-calpain in calcium-triggered apoptotic HeLa cells. PLoS One 9:e93642 (2014). Read more (PubMed: 24691096) »
  • Lachance G  et al. DNMT3a epigenetic program regulates the HIF-2a oxygen-sensing pathway and the cellular response to hypoxia. Proc Natl Acad Sci U S A 111:7783-8 (2014). Read more (PubMed: 24817692) »

See all 20 Publications for this product

Product Wall

Miss Sarah Sabir

Excellent Excellent 5/5 (Ease of Use)
Abreviews
U2OS cells were either treated with Staurosporine (0.5 uM) for 6 hours or left untreated in 6cm dishes. Cells were resuspended using PBS-EDTA and collected in a 15ml tube along with original culture media. Cells were centifuged at 1100rpm for 5 minutes and then resuspended in binding buffer before adding AnnexinV-FITC and PI as in protocol. Cells were incubated for 5 minutes at RT and then analysed.
Additional controls (graphs not shown): no stain, PI only, Annexin V only
Figure legend: U2OS cells were either A. Untreated, or B. Treated with 0.5uM Staurosporine for 6 hours. Percentage of Live (Q3), early apoptotic (Q4) and late apoptotic (Q2) populations are indicated.
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Ms. Sarah Sabir

Verified customer

Submitted Nov 26 2013

Regarding ab14085 Annexin V-FITC Apoptosis Detection Kit , I can confirm this assay can indeed be adapted to a plate reader format as well. For this particular assay, take 10^5 trypsinized cells into the plate wells, resuspend in 100 µl binding b...

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Thank you for your inquiry.

I can confirm that ab14082 (5X Annexin V-FITC Apoptosis Detection Reagent) and ab14084 (10X Annexin V Binding Buffer) are the same reagents as in the kit ab14085(Annexin V-FITC Apoptosis Detection Kit).

P...

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Thank you for contacting us.

I have had the opportunity to discuss this with the originator. Since this product has not yet been tested in this manner we are unsure whether it might work. We cannot rule this out but feel that a number of exp...

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Thank you for your email.

I discussed with my colleagues from the Scientific Support Team about the use of these kits in yeast and we agreed that the kit that is likely to work is the In situ Direct DNA Fragmentation Assay Kit (ab66108) but no...

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Thank you for contacting us.

Unfortunately, these kits have been standardized for mammalian cells and the lab do not have the protocol suggestion of how to fit these to yeast cells, since they have never tried that.

Sorry I couldn't b...

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Thank you for your call today and for letting us know about the trouble with this kit.

As we discussed, I'll be back in touch once I have more information about the QC procedures from the production lab.

I am sending a free of charg...

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Thank you for your inquiry.

Yes, this kit should work with all mammalian cells.

I hope this information is helpful. Please contact us with any other questions.

Merci de nous avoir contactés. Ma collègue Claudia est actuellement absente et je me permets donc de vous contacter.

Nos collaborateursqui ontdéveloppé ce kit nous ont informé que le marquage de l'annexine V...

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The trypsin used in this kit should in no way affect the PS on the cell membrane. I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"