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Annexin V Human ELISA kit (with color giving dyes) (ab119503)

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Overview

  • Product nameAnnexin V Human ELISA kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall 11%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich
  • Sensitivity
    0.33 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    85 %

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119503 is an enzyme-linked immunosorbent assay for the quantitative detection of Human Annexin V. The Human Annexin V ELISA is for research use only and is not for diagnostic or therapeutic procedures.

    Annexin V will bind to the phosphatidylserine-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. Total endogenous Annexin V can be detected with the present ELISA which will offer further insights in the pathophysiological role of Annexin V.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Anchorin CIIAnnexin A5Annexin V
        Annexin-5ANXA5ANXA5_HUMANCalphobindin ICBP-IEndonexin IILipocortin VPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPp4Thromboplastin inhibitorVAC-alphaVascular anticoagulant-alpha
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119503 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Annexin V Human ELISA kit (with color giving dyes) images

      • Sandwich ELISA - Annexin V Human ELISA kit (with color giving dyes) (ab119503)
        Sandwich ELISA - Annexin V Human ELISA kit (with color giving dyes) (ab119503)
        Representative standard curve using ab119503.

      Protocols

      References for Annexin V Human ELISA kit (with color giving dyes) (ab119503)

      ab119503 has not yet been referenced specifically in any publications.

      Publishing research using ab119503 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab119503 Submit a Question for ab119503

      Displaying 1 - 4 of 4 results for Abreviews and Q&A

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      With due respect, I want to tell you that my lab. Technician ordered 4 kits for me and I was busy to do my experiments.I have already done 48 samples and I am not getting result, and even I am getting less concentration of Annexin 5 than Blank wells. How ...

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      Thank you for your reply.
      please do let us know the details of your protocol, the steps you have performed as well as the raw annotated data, andwe will be pleased to look into this in more details.

      I look forward to your reply.

      Hello,



      Thanks for time to time cooperation. We Have already ordered 4 kits from your company which will come very soon and if we need individual componentfurther then we will let you know. As I am a masters thesis student, still I a...

      Read More

      Thank you very much for your inquiry.

      Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.
      We have not performed stability studies regarding the long -term storage of reconstitut...

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      What is the best way to store the standard after resuspension? How long can the standard be stored in this way?

      Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.


      We have not performed stability studies regarding the long -term storage of reconstituted standard protein so unfortunately ...

      Read More

      What are the reconstitution instructions for the standard in this Annexin V Human ELISA kit?

      Thank you for contacting us. The reconstitution instructions for the standard are lot specific and will be written on the vial. If for any reason it is not, please let us know your lot number and we will be happy to find that information for you.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"