anti-Annexin V Human ELISA kit (with color giving dyes) (ab119504)

Overview

• Product nameanti-Annexin V Human ELISA kit (with color giving dyes)
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Overall				2%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Overall				3.8%

• Tests
  1 x 96 test
• Sample type
  Cell culture supernatant, Serum
• Assay typeSandwich
• Sensitivity
  1.2 ng/ml
• Range
  6.3 ng/ml - 400 ng/ml
• Recovery

  86 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	86	74% - 100%
  Serum	86	74% - 100%

• Species reactivity
  Reacts with: Human
  
• Product overview

  ab119504 is an enzyme-linked immunosorbent assay for the quantitative detection of antibodies to Human Annexin V (Vac- alpha). The human anti-Annexin V ELISA is for research use only and is not for diagnostic or therapeutic procedures.

  Annexins are a family of calcium-dependent phospholipid-binding proteins. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates.

  Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel. Annexin V?s preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. These findings together with the presence of Annexin V in the extracellular space depict a novel pathophysiological significance for Annexin V in vivo.

  The occurrence of autoantibodies to annexin V has been described in several pathological disorders. It is concluded that extracellular annexin V provides an antigenic stimulus for autoantibody production. Such autoantibodies may have a detrimental role interfering with putative functions of annexin V.

  In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

• Tested applicationsSandwich ELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  Adhesive Films	2 units
  Assay Buffer Concentrate (20x)	1 x 5ml
  Blue-Dye	1 x 0.4ml
  Green-Dye	1 x 0.4ml
  HRP-Conjugate recombinant Annexin V	1 x 6ml
  Human anti-Annexin V Standard lyophilized	2 vials
  Recombinant Annexin V-coated microwell plate	1 unit
  Stop Solution	1 x 15ml
  Substrate Solution	1 x 15ml
  Wash Buffer Concentrate (20x)	1 x 50ml

• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Detection kits
  • Annexin V

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Apoptosis marker and proteins ELISA kits

  • Cardiovascular
  • Atherosclerosis
  • Apoptosis

  • Cell Biology
  • Apoptosis
  • Annexin V Reagents

Applications

anti-Annexin V Human ELISA kit (with color giving dyes) images

• 

  ELISA - anti-Annexin V Human ELISA kit (with color giving dyes) (ab119504)
  Representative standard curve using ab119504.

Protocols

Protocol Booklet