anti-Annexin V Human ELISA kit (with color giving dyes) (ab119504)
- Product nameanti-Annexin V Human ELISA kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Overall 2% Inter-assay Sample n Mean SD CV% Overall 3.8%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity1.2 ng/ml
- Range6.3 ng/ml - 400 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 86 74% - 100% Serum 86 74% - 100%
- Species reactivityReacts with: Human
- Product overview
ab119504 is an enzyme-linked immunosorbent assay for the quantitative detection of antibodies to Human Annexin V (Vac- alpha). The human anti-Annexin V ELISA is for research use only and is not for diagnostic or therapeutic procedures.
Annexins are a family of calcium-dependent phospholipid-binding proteins. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site. Thus annexin V seems to modulate the activity of some PKCs on their substrates.
Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel. Annexin V?s preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell. These findings together with the presence of Annexin V in the extracellular space depict a novel pathophysiological significance for Annexin V in vivo.
The occurrence of autoantibodies to annexin V has been described in several pathological disorders. It is concluded that extracellular annexin V provides an antigenic stimulus for autoantibody production. Such autoantibodies may have a detrimental role interfering with putative functions of annexin V.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 2 units Assay Buffer Concentrate (20x) 1 x 5ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml HRP-Conjugate recombinant Annexin V 1 x 6ml Human anti-Annexin V Standard lyophilized 2 vials Recombinant Annexin V-coated microwell plate 1 unit Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
Contains 4 annexin repeats.
- Anchorin CIIAnnexin A5Annexin V
- Annexin-5ANXA5ANXA5_HUMANCalphobindin ICBP-IEndonexin IILipocortin VPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPp4Thromboplastin inhibitorVAC-alphaVascular anticoagulant-alpha
Our Abpromise guarantee covers the use of ab119504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
anti-Annexin V Human ELISA kit (with color giving dyes) images
Representative standard curve using ab119504.
References for anti-Annexin V Human ELISA kit (with color giving dyes) (ab119504)
ab119504 has not yet been referenced specifically in any publications.