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Annexin V-PE Apoptosis Detection Kit (ab14155)

Q&A (4)Specific References (1)

Overview

  • Product nameAnnexin V-PE Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 test
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cyt more details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Anchorin CIIAnnexin 5Annexin A5
        Annexin VAnnexin-5Annexin5AnnexinA5AnnexinVANX 5ANX A5ANX5ANXA5ANXA5_HUMANCalphobindin ICBP ICBP-IEndonexin IIENX 2ENX2Lipocortin VPAP IPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPP 4Pp4RPRGL3Thromboplastin inhibitorVAC alphaVAC-alphaVascular anticoagulant alphaVascular anticoagulant-alpha
      see all

    Applications

    Our Abpromise guarantee covers the use of ab14155 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Flow Cyt Flow Cyt

    Protocols

    References for Annexin V-PE Apoptosis Detection Kit (ab14155)

    This product has been referenced in:

    See 1 Publication for this product

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    Submit an Abreview for ab14155 Submit a Question for ab14155

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    I had a query about Annexin V –PE kit Ab14155. In the attached data sheet the components are listed as Annexin V-PE/Cy5 rather than Annexin-PE. What is annexin V conjugated to in this kit?

    Regards

    Thank you for your enquiry.

    I have investigated our records for ab14155. I can confirm that this kit contains Annexin V-PE.

    The datasheet has been updated with the correct information and I apologize for any confusion. (Please note i...

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    I have recently purchased yourAnnexin V-PE Apoptosis Detection Kit (ab14155) to assay T cell apoptosis. For the assay I need an isotype control, however the data sheet does not mention what type of antibody the Annexin-V antibody is (e.g. mouse IgG1 ...

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    Thanks for your enquiry.

    The reagent that comes with this kit is not an antibody. It is PE/Cy5-labeled Annexin V protein. Annexin V will bind specifically to the membrane phospholipid phosphatidylserine, which in apoptotic cells has translocated...

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    Dear Sir, Thank you for your kind help. I have an inquiry about your product, ab14155; Annexin V-PE Apoptosis Detection Kit. One of our customers used it with home-made 1X Binding buffer and all of cells died. The formulation of hom-made buffer is as ...

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    The source of the Annexin V-PE Apoptosis Detection Kit informed me that the formulation of the buffer is confidential and therefore I am unable to compare it with the customer's home made buffer, I'm very sorry. The customer can buy more of the buffer ...

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    We have been using Annexin V-PE kit (ab14155) and run out of the binding buffer. Is the buffer in this kit sold separately? Does your product annexin V 10X binding buffer (ab140804) work with this kit? We appreciate your help if you could respond it ...

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    Thank you for your enquiry. Yes, ab14084 is the replacement buffer product for this kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"