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The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
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|Annexin V-PE||1 x 500µl|
|Binding Buffer||1 x 50ml|
Our Abpromise guarantee covers the use of ab14155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent dilution.|
Annexin V detected in 10e4 control Jurkat cells (black) or in cells treated with 50 μM H2O2 (Sigma) for 6 hours (red).
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