Overview

  • Product nameAnnexin V-PE Apoptosis Detection Kit
    See all Annexin V kits
  • Tests
    100 x 1 test
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    The Annexin V-PE Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cytmore details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-PE Apoptosis Detection Kit (ab14155)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your enquiry.

I have investigated our records for ab14155. I can confirm that this kit contains Annexin V-PE.

The datasheet has been updated with the correct information and I apologize for any confusion. (Please not...

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Thanks for your enquiry.

The reagent that comes with this kit is not an antibody. It is PE/Cy5-labeled Annexin V protein. Annexin V will bind specifically to the membrane phospholipid phosphatidylserine, which in apoptotic cells has transloca...

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The source of the Annexin V-PE Apoptosis Detection Kit informed me that the formulation of the buffer is confidential and therefore I am unable to compare it with the customer's home made buffer, I'm very sorry. The customer can buy more of the buff...

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Thank you for your enquiry. Yes, ab14084 is the replacement buffer product for this kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"