Annexin V-PE-Cy5 Apoptosis Detection Kit (ab14159)

Overview

  • Product nameAnnexin V-PE-Cy5 Apoptosis Detection KitSee all Annexin V kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeDirect
  • Assay time
    0h 10m
  • Product overview

    Abcam's Annexin V-PE-Cy5 Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFluorescence Microscopy, Flow Cytmore details

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all

Applications

Our Abpromise guarantee covers the use of ab14159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-PE-Cy5 Apoptosis Detection Kit (ab14159)

ab14159 has not yet been referenced specifically in any publications.

Product Wall



We do not have any experimentaldata for theuse of this kitwith GFP, howeverI think this should be ok sincethe kit uses the tandem (PE-Cy5) for which the excitation wavelengthis 488 nm and the emission 670nm. This should not interfere with the...

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I'm very sorry to hear you are having problems with this kit and for the shortages you have experienced. I have arranged for 2 kits to be sent to you as soon as possible (order reference 80894) and apologise for the inconvenience. I also enclose the ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"