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Annexin V-PE-Cy5 Apoptosis Detection Reagent (ab14158)

Submit an Abreview Q&A (4)Specific References (2)

Overview

  • Product nameAnnexin V-PE-Cy5 Apoptosis Detection ReagentSee all Annexin V reagents ...
  • Tested applicationsFluorescence Microscopy, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • General notes

    Annexin V-PE-Cy5 is a bright fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+ dependent affinity for PS and therefore serves as a probe for detecting apoptosis.

    • Properties

  • FormLiquid
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, 0.25mg/ml PBS
  • Concentration information loading...
  • Research Areas
  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Anchorin CIIAnnexin 5Annexin A5
        Annexin VAnnexin-5Annexin5AnnexinA5AnnexinVANX 5ANX A5ANX5ANXA5ANXA5_HUMANCalphobindin ICBP ICBP-IEndonexin IIENX 2ENX2Lipocortin VPAP IPAP-IPlacental anticoagulant protein 4Placental anticoagulant protein IPP 4Pp4RPRGL3Thromboplastin inhibitorVAC alphaVAC-alphaVascular anticoagulant alphaVascular anticoagulant-alpha
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab14158 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Fluorescence Microscopy Fluorescence Microscopy: Use at an assay dependent concentration.
      Flow Cyt Flow Cyt: Use at an assay dependent dilution.

      Protocols

      References for Annexin V-PE-Cy5 Apoptosis Detection Reagent (ab14158)

      This product has been referenced in:
      • Naka K  et al. TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia. Nature 463:676-80 (2010). ICC/IF ; Mouse . Read more (PubMed: 20130650) »
      • Shah MD  et al. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets 19:365-72 (2008). Read more (PubMed: 18791943) »

      See all 2 Publications for this product

      Publishing research using ab14158 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab14158 Submit a Question for ab14158

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      Has this product been tested with mouse cells?

      Thank you for your enquiry. Annexin V apoptosis kits work well with all kinds of mammalian species. The assay has no species preference. I hope this information helps. Please do not hesitate to contact us if you need anything further.

      Does this antibody work for adherent cells?

      Thank you for your enquiry. Yes, this reagent will work for adherent cells for either FACS or fluorescence microscopy. For FACS, you would simply trypsinize the cells and wash once in medium before proceeding with the protocol. The protocol used with...

      Read More

      What are the excitation and emission wavelengths for this product?

      Thank you for your enquiry. The following wavelengths should be used: (Ex = 488 nm; Em = 670 nm). If you have any additional questions, please contact us again.

      Customer would like to know: what is the concentration of Annexin V in this reagent?

      The concentration is 250 ug/ml.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"