Annexin V-PE-Cy5 Apoptosis Detection Reagent (ab14158)

Overview

  • Product nameAnnexin V-PE-Cy5 Apoptosis Detection ReagentSee all Annexin V reagents ...
  • Tested applicationsFluorescence Microscopy, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • General notes

    Annexin V-PE-Cy5 is a bright fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+ dependent affinity for PS and therefore serves as a probe for detecting apoptosis.

Properties

  • FunctionThis protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
  • Sequence similaritiesBelongs to the annexin family.
    Contains 4 annexin repeats.
  • DomainA pair of annexin repeats may form one binding site for calcium and phospholipid.
  • Target information above from: UniProt accession P08758 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Anchorin CII
    • Annexin 5
    • Annexin A5
    • Annexin V
    • Annexin-5
    • Annexin5
    • AnnexinA5
    • AnnexinV
    • ANX 5
    • ANX A5
    • ANX5
    • ANXA5
    • ANXA5_HUMAN
    • Calphobindin I
    • CBP I
    • CBP-I
    • Endonexin II
    • ENX 2
    • ENX2
    • Lipocortin V
    • PAP I
    • PAP-I
    • Placental anticoagulant protein 4
    • Placental anticoagulant protein I
    • PP 4
    • Pp4
    • RPRGL3
    • Thromboplastin inhibitor
    • VAC alpha
    • VAC-alpha
    • Vascular anticoagulant alpha
    • Vascular anticoagulant-alpha
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab14158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Fluorescence Microscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Annexin V-PE-Cy5 Apoptosis Detection Reagent (ab14158)

This product has been referenced in:
  • Naka K  et al. TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia. Nature 463:676-80 (2010). ICC/IF ; Mouse . Read more (PubMed: 20130650) »
  • Shah MD  et al. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets 19:365-72 (2008). Read more (PubMed: 18791943) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. Annexin V apoptosis kits work well with all kinds of mammalian species. The assay has no species preference. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. Yes, this reagent will work for adherent cells for either FACS or fluorescence microscopy. For FACS, you would simply trypsinize the cells and wash once in medium before proceeding with the protocol. The protocol used w...

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Thank you for your enquiry. The following wavelengths should be used: (Ex = 488 nm; Em = 670 nm). If you have any additional questions, please contact us again.

The concentration is 250 ug/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"