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- Secondary antibodies
- Lysates
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Read our guarantee »Overview
Product name
Anti-Annexin V antibody
See all Annexin V products (12) ...
Description
Rabbit polyclonal to Annexin V
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Chimpanzee
Immunogen
A reason within synthetic peptide: KSELTGKFEK LIVALMKPSR LYDAYELKHA LKGAGTNEKV LTEIIASRTP, corresponding to N terminal amino acids 70-119 of Human Annexin V
KSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKV LTEIIASRTP
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Detection kits >> Annexin V
Cell Biology >> Apoptosis >> Annexin V Reagents
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa).Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats.
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid.
Target information above from: UniProt accessionP08758
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anchorin CII antibody
- Annexin 5 antibody
- Annexin A5 antibody
see all
Database links
- Entrez Gene: 308 Human
- Entrez Gene: 11747 Mouse
- Entrez Gene: 25673 Rat
- Omim: 131230 Human
- SwissProt: P08758 Human
- SwissProt: P48036 Mouse
- SwissProt: P14668 Rat
- Unigene: 480653 Human
see all
Anti-Annexin V antibody images:
Western blot - Annexin V antibody (ab30941)
Anti-Annexin V antibody (ab30941) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 36 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Western blot - Anti-Annexin V antibody (ab30941)
Anti-Annexin V antibody (ab30941) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 seconds
References for Anti-Annexin V antibody (ab30941)
This product has been referenced in:
- Grzendowski M et al. Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q. Neuro Oncol 12:243-56 (2010). WB; Human.Read more (PubMed: 20167812) »
See 1 publication for this product
Publishing research using ab30941? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"