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Annexin VII 293T Transfected Lysate - (positive control) (ab94175)

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Overview

  • Product nameAnnexin VII 293T Transfected Lysate - (positive control)
  • General notesab94175 is a 293T cell transfected lysate in which Human Annexin VII has been transiently over-expressed using a pCMV-Annexin VII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Tissue specificity: Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Applications

    Our Abpromise guarantee covers the use of ab94175 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Annexin VII 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Annexin VII 293T Transfected Lysate - (positive control) (ab94175)
      SDS-PAGE - Annexin VII 293T Transfected Lysate - (positive control) (ab94175)
      ab94175 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Annexin VII 293T Transfected Lysate - (positive control) (ab94175)
      Western blot - Annexin VII 293T Transfected Lysate - (positive control) (ab94175)
      All lanes : Anti-Annexin VII antibody (ab88539) at 1/500 dilution

      Lane 1 : Annexin VII 293T Transfected Lysate - (positive control) (ab94175)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Annexin VII 293T Transfected Lysate - (positive control) (ab94175)

    ab94175 has not yet been referenced specifically in any publications.

    Publishing research using ab94175 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"