Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)

Overview

  • Product nameAnnexin VIII 293T Transfected Lysate - (positive control)
  • General notesab94176 is a 293T cell transfected lysate in which Human Annexin VIII has been transiently over-expressed using a pCMV-Annexin VIII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

Applications

Our Abpromise guarantee covers the use of ab94176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Annexin VIII 293T Transfected Lysate - (positive control) images

    • ab94176 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-Annexin VIII antibody (ab73876) at 1/500 dilution

      Lane 1 : Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)

    ab94176 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"