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Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)

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Overview

  • Product nameAnnexin VIII 293T Transfected Lysate - (positive control)
  • General notesab94176 is a 293T cell transfected lysate in which Human Annexin VIII has been transiently over-expressed using a pCMV-Annexin VIII plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

    • Applications

    Our Abpromise guarantee covers the use of ab94176 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Annexin VIII 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      SDS-PAGE - Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      ab94176 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      Western blot - Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      All lanes : Anti-Annexin VIII antibody (ab73876) at 1/500 dilution

      Lane 1 : Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Annexin VIII 293T Transfected Lysate - (positive control) (ab94176)

    ab94176 has not yet been referenced specifically in any publications.

    Publishing research using ab94176 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"