Annexin-8/ANXA8 overexpression 293T lysate (whole cell) (ab94176)
Overview
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Product name
Annexin-8/ANXA8 overexpression 293T lysate (whole cell) -
General notes
ab94176 is a 293T cell transfected lysate in which Human Annexin-8/ANXA8 has been transiently over-expressed using a pCMV-Annexin-8/ANXA8 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Research areas
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Background
Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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Notes |
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WB
Use at an assay dependent dilution. |
Images
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ab94176 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Annexin-8/ANXA8 antibody (ab73876) at 1/500 dilution
Lane 1 :Annexin-8/ANXA8 overexpression 293T lysate (whole cell) (ab94176)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab94176 has not yet been referenced specifically in any publications.