Anti-Anoctamin 7 antibody - C-terminal (ab196675)
Key features and details
- Rabbit polyclonal to Anoctamin 7 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Anoctamin 7 antibody - C-terminal -
Description
Rabbit polyclonal to Anoctamin 7 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Anoctamin 7 (C terminal).
Database link: Q6IWH7 -
Positive control
- LOVO cell extract; Human prostrate carcinoma tissue; HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab196675 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 106 kDa.
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Notes |
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ICC/IF
1/100 - 1/500. |
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 106 kDa. |
Target
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Function
Does not exhibit calcium-activated chloride channel (CaCC) activity. May play a role in cell-cell interactions. -
Tissue specificity
Specifically expressed in epithelial cells of the prostate (at protein level). -
Sequence similarities
Belongs to the anoctamin family. -
Cellular localization
Cytoplasm > cytosol and Cell membrane. Cell junction. Endoplasmic reticulum. Concentrates at sites of cell-cell contact. Shows an intracellular localization according to PubMed:22075693 and PubMed:20056604. - Information by UniProt
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Database links
- Entrez Gene: 50636 Human
- Omim: 605096 Human
- SwissProt: Q6IWH7 Human
- Unigene: 163909 Human
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Alternative names
- Ano7 antibody
- ANO7_HUMAN antibody
- Anoctamin 7 antibody
see all
Images
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All lanes : Anti-Anoctamin 7 antibody - C-terminal (ab196675) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunizing peptide
Predicted band size: 106 kDa -
Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling Anoctamin 7 with ab196675 at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
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Immunofluorescent analysis of HepG2 cells labeling Anoctamin 7 with ab196675 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196675 has not yet been referenced specifically in any publications.