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All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
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