Overview

  • Product nameAnti-Anterior Gradient 2 antibody [EPR3278]
    See all Anterior Gradient 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3278] to Anterior Gradient 2
  • Tested applicationsWB, IP, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in human Anterior Gradient 2.

  • Positive control
    • HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to DyLight® 488 see ab139894.

Properties

Applications

Our Abpromise guarantee covers the use of ab76473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 20 kDa.
IP 1/20.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

Target

  • FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • Sequence similaritiesBelongs to the AGR family.
  • Cellular localizationSecreted. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HPC 8 antibody
    • HPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all

Anti-Anterior Gradient 2 antibody [EPR3278] images

  • All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution

    Lane 1 : HT-29 cell lysates
    Lane 2 : MCF-7 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 20 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  • ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
  • ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.

References for Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)

This product has been referenced in:
  • Li Y  et al. N,N'-dinitrosopiperazine-mediated AGR2 is involved in metastasis of nasopharyngeal carcinoma. PLoS One 9:e92081 (2014). IP, IHC-P ; Human . Read more (PubMed: 24717913) »
  • Lee HJ  et al. Identification of novel HLA-A*0201-restricted epitopes from anterior gradient-2 as a tumor-associated antigen against colorectal cancer. Cell Mol Immunol 9:175-83 (2012). Read more (PubMed: 22231555) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"