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Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/ps/datasheet/images/ab76473_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/ps/datasheet/images/ab76473-2.gif)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/ps/datasheet/images/ab76543-3.gif)
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Product Name
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Anterior Gradient 2 antibody [EPR3278]
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See all Anterior Gradient 2 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3278] to Anterior Gradient 2
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Immunogen
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Synthetic peptide corresponding to residues in human Anterior Gradient 2.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/20.
ICC: 1/100 - 1/250.
IHC-P: 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: 1/20.
WB: 1/5000 - 1/10000. Predicted molecular weight: 20 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma.
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Cellular localization
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Secreted
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Lineage Markers >> Ectoderm
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Relevance
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Anterior gradient 2 (AGR2) and Anterior gradient 3 (AGR3) are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Both AGR2 and AGR3 share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. AGR2 is expressed in a range of tumors including lung and breast tumors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3278
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Anterior Gradient 2 antibody [EPR3278] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Anterior Gradient 2 antibody [EPR3278] images, references, products related to ab76473 and other tools.
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Anterior Gradient 2 antibody [EPR3278] images:
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 Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)
All lanes : Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
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Search PubMed (MEDLINE) for references to Anterior Gradient 2
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Anterior Gradient 2 antibody [EPR3278] - more information
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Customer reviews (feedback) regarding Anterior Gradient 2 antibody [EPR3278] |
Customer FAQs regarding Anterior Gradient 2 antibody [EPR3278] |
Protocols for Anterior Gradient 2 antibody [EPR3278] |
Price and availability of products related to Anterior Gradient 2 antibody [EPR3278] |
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Anterior Gradient 2 antibody [EPR3278] - related products:
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Anterior Gradient 2 antibody [EPR3278] - other tools:
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Contact Abcam with a Technical Enquiry about Anterior Gradient 2 antibody [EPR3278] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/index.html?datasheet=76473#ab76473_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/index.html?datasheet=76473#ab76473-2.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)](/index.html?datasheet=76473#ab76543-3.gif)
