Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)

Overview

• Product nameAnti-Anterior Gradient 2 antibody [EPR3278]See all Anterior Gradient 2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3278] to Anterior Gradient 2
• Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues in human Anterior Gradient 2.

• Positive controlHT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3278
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Tumor biomarkers
  • Other

Applications

Target

• FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
• Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
• Sequence similaritiesBelongs to the AGR family.
• Cellular localizationSecreted. Endoplasmic reticulum.

Target information above from: UniProt accession O95994 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10551 Human
  • Omim: 606358 Human
  • SwissProt: O95994 Human
  • Unigene: 530009 Human

• Alternative names
  AG 2 antibodyAG 2 protein antibodyAG-2 antibody

  AG2 antibodyAG2 protein antibodyAGR 2 antibodyAGR2 antibodyAGR2_HUMAN antibodyAnterior gradient 2 homolog (Xenopus laevis) antibodyanterior gradient 2 homolog antibodyAnterior gradient homolog 2 antibodyAnterior gradient protein 2 homolog antibodyGOB 4 antibodyGOB4 antibodyHAG 2 antibodyhAG-2 antibodyhAG2 antibodyHAG2/R antibodyHPC 8 antibodyHPC8 antibodyPDIA17 antibodyProtein disulfide isomerase family A member 17 antibodysecreted cement gland homolog antibodySecreted cement gland protein XAG 2 homolog antibodySecreted cement gland protein XAG-2 homolog antibodySecreted cement gland protein XAG2 homolog antibodyXAG 2 antibodyXAG2 antibody

  see all

Anti-Anterior Gradient 2 antibody [EPR3278] images

• 

  Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)
  All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
  
  Lane 1 : HT-29 cell lysates
  Lane 2 : MCF-7 cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 20 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
  ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
  ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.