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Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)

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Overview

Product name

Anti-Anterior Gradient 2 antibody [EPR3278]
See all Anterior Gradient 2 products (8) ...

Description

Rabbit monoclonal [EPR3278] to Anterior Gradient 2

Tested applications

WB, IP, IHC-P, ICC, Flow Cytmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

Synthetic peptide corresponding to residues in human Anterior Gradient 2.

Positive control

HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR3278

Isotype

IgG

  • Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/5000 - 1/10000.Pre...Read more →

    WB: 1/5000 - 1/10000.Predicted molecular weight: 20 kDa.

  • IP

     IP: 1/20

    IP: 1/20

  • ShowHide2 Images

    IHC-P

     IHC-P: 1/250 - 1/500.Per...Read more →

    IHC-P: 1/250 - 1/500.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ICC

     ICC: 1/100 - 1/250.

    ICC: 1/100 - 1/250.

  • Flow Cyt

     Flow Cyt: 1/20

    Flow Cyt: 1/20

Target

Function

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.

Tissue specificity

Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.

Sequence similarities

Belongs to the AGR family.

Cellular localization

Secreted. Endoplasmic reticulum.

Target information above from: UniProt accessionO95994 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AG2 protein antibody
  • hAG2 antibody
  • Secreted cement gland protein XAG2 homolog antibody
  • AG 2 antibody
  • AG 2 protein antibody
  • AG-2 antibody
  • AG2 antibody
  • AGR 2 antibody
  • AGR2 antibody
  • AGR2_HUMAN antibody
  • Anterior gradient 2 homolog (Xenopus laevis) antibody
  • anterior gradient 2 homolog antibody
  • Anterior gradient protein 2 homolog antibody
  • GOB 4 antibody
  • GOB4 antibody
  • HAG 2 antibody
  • hAG-2 antibody
  • HAG2/R antibody
  • HPC 8 antibody
  • HPC8 antibody
  • PDIA17 antibody
  • secreted cement gland homolog antibody
  • Secreted cement gland protein XAG 2 homolog antibody
  • Secreted cement gland protein XAG-2 homolog antibody
  • XAG 2 antibody
  • XAG2 antibody
see all

Anti-Anterior Gradient 2 antibody [EPR3278] images:

  Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)

Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)

All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution

Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)

ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)

ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.

References for Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)

ab76473 has not yet been referenced specifically in any publications.

Publishing research using ab76473? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"