![Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](#ab76473_1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](#ab76473-2.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](#ab76543-3.gif)
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Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
Anti-Anterior Gradient 2 antibody [EPR3278]
See all Anterior Gradient 2 products (8) ...
Description
Rabbit monoclonal [EPR3278] to Anterior Gradient 2
Tested applications
WB, IP, IHC-P, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in human Anterior Gradient 2.
Positive control
HT-29 and MCF-7 cell lysates. Human colon tissue. Human endometrium adenocarcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3278
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Lineage Markers >> Ectoderm
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Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](/ps/datasheet/Images/76/ab76473/ab76473_1.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](/ps/datasheet/Images/76/ab76473/ab76473-2.gif)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473) image](/ps/datasheet/Images/76/ab76473/ab76543-3.gif)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/10000.Predicted molecular weight: 20 kDa.
IP
IP: 1/20IP: 1/20
IHC-P: 1/250 - 1/500.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt
Flow Cyt: 1/20Flow Cyt: 1/20
Target
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Tissue specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Sequence similarities
Belongs to the AGR family.
Cellular localization
Secreted. Endoplasmic reticulum.
Target information above from: UniProt accessionO95994
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AG2 protein antibody
- hAG2 antibody
- Secreted cement gland protein XAG2 homolog antibody
see all
Database links
- Entrez Gene: 10551 Human
- Omim: 606358 Human
- SwissProt: O95994 Human
- Unigene: 530009 Human
Anti-Anterior Gradient 2 antibody [EPR3278] images:
Western blot - Anterior Gradient 2 antibody [EPR3278] (ab76473)
All lanes : Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473) at 1/10000 dilution
Lane 1 : HT-29 cell lysates
Lane 2 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anterior Gradient 2 antibody [EPR3278] (ab76473)
ab76473, at 1/500 dilution, staining Anterior Gradient 2 in paraffin-embedded human endometrium adenocarcinoma by Immunohistochemistry.
References for Anti-Anterior Gradient 2 antibody [EPR3278] (ab76473)
ab76473 has not yet been referenced specifically in any publications.
Publishing research using ab76473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"