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Products:Stem Cells >> Lineage Markers >> Ectoderm
Overview
Product name
Anti-Anterior Gradient 2 antibody
See all Anterior Gradient 2 products (8) ...
Description
Rabbit polyclonal to Anterior Gradient 2
Specificity
ab22208 reacts with Anterior Gradient 2 (AGR2) Homolog.
Tested applications
ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee
Immunogen
Synthetic peptide: TQTYEEALYK SKTSNKPL, corresponding to internal sequence amino acids 55-72 of Human Anterior Gradient 2.
TQTYEEALYK SKTSNKPL
Positive control
Normal colon tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Lineage Markers >> Ectoderm
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Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab22208)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
IHC-P
ICC/IF
Application notes
ELISA: 1/100 - 1/1000.
ICC/IF: 1/200 (PMID 19359471).
IHC-P: 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Tissue specificity
Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Sequence similarities
Belongs to the AGR family.
Cellular localization
Secreted. Endoplasmic reticulum.
Target information above from: UniProt accessionO95994
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AG2 protein antibody
- hAG2 antibody
- Secreted cement gland protein XAG2 homolog antibody
see all
Database links
- Entrez Gene: 420596 Chicken
- Entrez Gene: 415112 Cow
- Entrez Gene: 482333 Dog
- Entrez Gene: 10551 Human
- Entrez Gene: 23795 Mouse
- Entrez Gene: 298961 Rat
- Omim: 606358 Human
- SwissProt: O95994 Human
see all
Anti-Anterior Gradient 2 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - Anterior Gradient 2 antibody (ab22208)
Immunohistochemical staining with ab22208 in normal colon tissue at 1:100 dilution.
References for Anti-Anterior Gradient 2 antibody (ab22208)
This product has been referenced in:
- Dumartin Let al. AGR2 is a novel surface antigen that promotes the dissemination of pancreatic cancer cells through regulation of cathepsins B and D. Cancer Res 71:7091-102 (2011).Read more (PubMed: 21948970) »
- Park SWet al. The protein disulfide isomerase AGR2 is essential for production of intestinal mucus. Proc Natl Acad Sci U S A 106:6950-5 (2009). ICC/IF, IHC-P; Mouse.Read more (PubMed: 19359471) »
See all 2 publications for this product
Publishing research using ab22208? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"