Anti-Anterior Gradient 2 antibody (ab42765)

Overview

  • Product nameAnti-Anterior Gradient 2 antibodySee all Anterior Gradient 2 primary antibodies ...
  • Description
    Rabbit polyclonal to Anterior Gradient 2
  • Tested applicationsWB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Anterior Gradient 2.

    (Peptide available as ab45078.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: MCF7 whole cell lysate, Rat Small Intestine, Mouse Small Intestine

Properties

Applications

Our Abpromise guarantee covers the use of ab42765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Anterior Gradient 2 peptide (ab45078).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
  • Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
  • Sequence similaritiesBelongs to the AGR family.
  • Cellular localizationSecreted. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AG 2 antibody
    • AG 2 protein antibody
    • AG-2 antibody
    • AG2 antibody
    • AG2 protein antibody
    • AG2 protein antibody
    • AGR 2 antibody
    • AGR2 antibody
    • AGR2_HUMAN antibody
    • Anterior gradient 2 homolog (Xenopus laevis) antibody
    • anterior gradient 2 homolog antibody
    • Anterior gradient 2, Xenopus, homolog of antibody
    • Anterior gradient homolog 2 antibody
    • Anterior gradient protein 2 homolog antibody
    • GOB 4 antibody
    • GOB4 antibody
    • HAG 2 antibody
    • hAG-2 antibody
    • hAG2 antibody
    • hAG2 antibody
    • HAG2/R antibody
    • HPC 8 antibody
    • HPC8 antibody
    • PDIA17 antibody
    • Protein disulfide isomerase family A member 17 antibody
    • secreted cement gland homolog antibody
    • Secreted cement gland protein XAG 2 homolog antibody
    • Secreted cement gland protein XAG-2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • Secreted cement gland protein XAG2 homolog antibody
    • XAG 2 antibody
    • XAG2 antibody
    see all

Anti-Anterior Gradient 2 antibody images

  • All lanes : Anti-Anterior Gradient 2 antibody (ab42765) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Small Intestine (Mouse) Tissue Lysate
    Lane 3 : Small Intestine (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab42765 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42765, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Anterior Gradient 2 antibody (ab42765)

ab42765 has not yet been referenced specifically in any publications.

Product Wall

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Application Western blot
Sample Mouse Cell lysate - whole cell (P388D1, macrophages)
Loading amount 10 µg
Specification P388D1, macrophages
Gel Running Conditions Reduced Denaturing (17%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (epithelial colorectal adenocarcinoma cells (Caco))
Loading amount 20 µg
Specification epithelial colorectal adenocarcinoma cells (Caco)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted May 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"