Overview
- Product nameAnti-Anterior Gradient 2 antibodySee all Anterior Gradient 2 primary antibodies ...
- DescriptionRabbit polyclonal to Anterior Gradient 2
- Tested applicationsWB, IHC-P, ICC/IF more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Orangutan - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Anterior Gradient 2.
(Peptide available as ab45078.)
- Positive controlThis antibody gave a positive signal in the following lysates: PC3 Whole Cell, LNCap Nuclear, PANC-1 Nuclear, Mouse Lung, Mouse Testis
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab42765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Anterior Gradient 2 peptide (ab45078). |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: Use a concentration of 5 µg/ml. |
Target
- FunctionRequired for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
- Tissue specificityExpressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
- Sequence similaritiesBelongs to the AGR family.
- Cellular localizationSecreted. Endoplasmic reticulum.
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Database links
- Entrez Gene: 415112 Cow
- Entrez Gene: 10551 Human
- Entrez Gene: 23795 Mouse
- Omim: 606358 Human
- SwissProt: O95994 Human
- SwissProt: O88312 Mouse
- SwissProt: Q5R7P1 Orangutan
- Unigene: 530009 Human
- Unigene: 7244 Mouse
see all
Target information above from: UniProt accession
O95994
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AG 2 antibodyAG 2 protein antibodyAG-2 antibody
- AG2 antibodyAG2 protein antibodyAG2 protein antibodyAGR 2 antibodyAGR2 antibodyAGR2_HUMAN antibodyAnterior gradient 2 homolog (Xenopus laevis) antibodyanterior gradient 2 homolog antibodyAnterior gradient homolog 2 antibodyAnterior gradient protein 2 homolog antibodyGOB 4 antibodyGOB4 antibodyHAG 2 antibodyhAG-2 antibodyhAG2 antibodyhAG2 antibodyHAG2/R antibodyHPC 8 antibodyHPC8 antibodyPDIA17 antibodyProtein disulfide isomerase family A member 17 antibodysecreted cement gland homolog antibodySecreted cement gland protein XAG 2 homolog antibodySecreted cement gland protein XAG-2 homolog antibodySecreted cement gland protein XAG2 homolog antibodySecreted cement gland protein XAG2 homolog antibodyXAG 2 antibodyXAG2 antibody
see all
Anti-Anterior Gradient 2 antibody images
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Western blot - Anterior Gradient 2 antibody (ab42765)All lanes : Anti-Anterior Gradient 2 antibody (ab42765) at 1 µg/ml
Lane 1 :PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)
Lane 2 :PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)
Lane 3 :LNCaP (Human prostate adenocarcinoma cell line) Nuclear Lysate - clone FGC (ab14857)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anterior Gradient 2 antibody (ab42765)All lanes : Anti-Anterior Gradient 2 antibody (ab42765) at 1 µg/ml
Lane 1 : Lung (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 :PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anterior Gradient 2 antibody (ab42765)ICC/IF image of ab42765 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42765, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Anterior Gradient 2 antibody (ab42765)
ab42765 has not yet been referenced specifically in any publications.
